1hr0: Difference between revisions

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-[[Image:1hr0.png|left|200px]]
-{{STRUCTURE_1hr0|  PDB=1hr0  |  SCENE=  }}
+==CRYSTAL STRUCTURE OF INITIATION FACTOR IF1 BOUND TO THE 30S RIBOSOMAL SUBUNIT==
+<StructureSection load='1hr0' size='340' side='right'caption='[[1hr0]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1hr0]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HR0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hr0 OCA], [https://pdbe.org/1hr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hr0 RCSB], [https://www.ebi.ac.uk/pdbsum/1hr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hr0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hr/1hr0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hr0 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Initiation of translation at the correct position on messenger RNA is essential for accurate protein synthesis. In prokaryotes, this process requires three initiation factors: IF1, IF2, and IF3. Here we report the crystal structure of a complex of IF1 and the 30S ribosomal subunit. Binding of IF1 occludes the ribosomal A site and flips out the functionally important bases A1492 and A1493 from helix 44 of 16S RNA, burying them in pockets in IF1. The binding of IF1 causes long-range changes in the conformation of H44 and leads to movement of the domains of 30S with respect to each other. The structure explains how localized changes at the ribosomal A site lead to global alterations in the conformation of the 30S subunit.
-===CRYSTAL STRUCTURE OF INITIATION FACTOR IF1 BOUND TO THE 30S RIBOSOMAL SUBUNIT===
+Crystal structure of an initiation factor bound to the 30S ribosomal subunit.,Carter AP, Clemons WM Jr, Brodersen DE, Morgan-Warren RJ, Hartsch T, Wimberly BT, Ramakrishnan V Science. 2001 Jan 19;291(5503):498-501. PMID:11228145<ref>PMID:11228145</ref>
-{{ABSTRACT_PUBMED_11228145}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1hr0" style="background-color:#fffaf0;"></div>
-[[1hr0]] is a 23 chain structure of [[Ribosomal protein S10]], [[Ribosomal protein S11]], [[Ribosomal protein S12]], [[Ribosomal protein S13]], [[Ribosomal protein S14]], [[Ribosomal protein S15]], [[Ribosomal protein S16]], [[Ribosomal protein S17]], [[Ribosomal protein S18]], [[Ribosomal protein S19]], [[Ribosomal protein S2]], [[Ribosomal protein S20]], [[Ribosomal protein S3]], [[Ribosomal protein S4]], [[Ribosomal protein S5]], [[Ribosomal protein S6]], [[Ribosomal protein S7]], [[Ribosomal protein S8]], [[Ribosomal protein S9]], [[Ribosomal protein THX]] and [[Ribosome]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HR0 OCA].
 ==See Also==
-*[[Ribosomal protein S10|Ribosomal protein S10]]
+*[[Ribosome 3D structures|Ribosome 3D structures]]
-*[[Ribosomal protein S11|Ribosomal protein S11]]
+== References ==
-*[[Ribosomal protein S12|Ribosomal protein S12]]
+<references/>
-*[[Ribosomal protein S13|Ribosomal protein S13]]
+__TOC__
-*[[Ribosomal protein S14|Ribosomal protein S14]]
+</StructureSection>
-*[[Ribosomal protein S15|Ribosomal protein S15]]
+[[Category: Large Structures]]
-*[[Ribosomal protein S16|Ribosomal protein S16]]
-*[[Ribosomal protein S17|Ribosomal protein S17]]
-*[[Ribosomal protein S18|Ribosomal protein S18]]
-*[[Ribosomal protein S19|Ribosomal protein S19]]
-*[[Ribosomal protein S2|Ribosomal protein S2]]
-*[[Ribosomal protein S20|Ribosomal protein S20]]
-*[[Ribosomal protein S3|Ribosomal protein S3]]
-*[[Ribosomal protein S4|Ribosomal protein S4]]
-*[[Ribosomal protein S5|Ribosomal protein S5]]
-*[[Ribosomal protein S6|Ribosomal protein S6]]
-*[[Ribosomal protein S7|Ribosomal protein S7]]
-*[[Ribosomal protein S8|Ribosomal protein S8]]
-*[[Ribosomal protein S9|Ribosomal protein S9]]
-*[[Ribosomal protein THX|Ribosomal protein THX]]
-*[[Ribosome|Ribosome]]
-==Reference==
-<ref group="xtra">PMID:011228145</ref><ref group="xtra">PMID:015502846</ref><ref group="xtra">PMID:015630477</ref><references group="xtra"/>
-[[Category: Escherichia coli]]
 [[Category: Thermus thermophilus]]
-[[Category: Brodersen, D E.]]
+[[Category: Brodersen DE]]
-[[Category: Carter, A P.]]
+[[Category: Carter AP]]
-[[Category: Clemons, W M.]]
+[[Category: Clemons Jr WM]]
-[[Category: Morgan-Warren, R J.]]
+[[Category: Morgan-Warren RJ]]
-[[Category: Ramakrishnan, V.]]
+[[Category: Ramakrishnan V]]
-[[Category: Wimberly, B T.]]
+[[Category: Wimberly BT]]
-[[Category: 30]]
-[[Category: If1]]
-[[Category: Initiation factor]]
-[[Category: Ribosomal subunit]]
-[[Category: Ribosome]]