1bwd: Difference between revisions

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-{{Seed}}
-[[Image:1bwd.png|left|200px]]
-<!--
+==INOSAMINE-PHOSPHATE AMIDINOTRANSFERASE STRB1 FROM STREPTOMYCES GRISEUS==
-The line below this paragraph, containing "STRUCTURE_1bwd", creates the "Structure Box" on the page.
+<StructureSection load='1bwd' size='340' side='right'caption='[[1bwd]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1bwd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BWD FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwd OCA], [https://pdbe.org/1bwd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bwd RCSB], [https://www.ebi.ac.uk/pdbsum/1bwd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwd ProSAT]</span></td></tr>
-{{STRUCTURE_1bwd|  PDB=1bwd  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/STRB1_STRGR STRB1_STRGR] Catalyzes two non-consecutive transamidination reactions. It converts scyllo-inosamine 4-phosphate into N-amidino-scyllo-inosamine 4-phosphate and N1-amidinostreptamine 6-phosphate into streptidine 6-phosphate.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/1bwd_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bwd ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Inosamine-phosphate amidinotransferases catalyze two nonconsecutive transamidination reactions in the biosynthesis of the streptomycin family of antibiotics. L-Arginine:inosamine-phosphate amidinotransferase StrB1 from Streptomyces griseus (StrB1) was cloned as an N-terminal hexa-histidine fusion protein, purified by affinity chromatography, and crystallized, and its crystal structure was solved by Patterson search methods at 3.1 A resolution. The structure is composed of five betabeta alphabeta-modules which are arranged circularly into a pseudo-5-fold symmetric particle. The three-dimensional structure is closely related to the structure of human L-arginine:glycine amidinotransferase (AT), but five loops (the 40-, 170-, 220-, 250-, and 270-loop) are organized very differently. The major changes are found in loops around the active site which open the narrow active site channel of AT to form an open and solvent-exposed cavity. In particular, module II of StrB1 is AT-like but lacks a 10-residue alpha-helix in the 170-loop. The concomitant reorganization of neighboring surface loops that surround the active site, i.e., the 40-loop and the 270-loop, results in an arrangement of loops which allows an unrestricted access of substrates to the cavity. However, the residues which are involved in substrate binding and catalysis are conserved in AT and StrB1 and are at equivalent topological positions, suggesting a similar reaction mechanism among amidinotransferases. The binding site for L-arginine had been deduced from its complex with AT. Molecular modeling revealed a possible binding mode for the second substrate scyllo-inosamine 4-phosphate.
-===INOSAMINE-PHOSPHATE AMIDINOTRANSFERASE STRB1 FROM STREPTOMYCES GRISEUS===
+Crystal structure of L-arginine:inosamine-phosphate amidinotransferase StrB1 from Streptomyces griseus: an enzyme involved in streptomycin biosynthesis.,Fritsche E, Bergner A, Humm A, Piepersberg W, Huber R Biochemistry. 1998 Dec 22;37(51):17664-72. PMID:9922132<ref>PMID:9922132</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_9922132}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1bwd" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 9922132 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_9922132}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-BWD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWD OCA].
-==Reference==
-Crystal structure of L-arginine:inosamine-phosphate amidinotransferase StrB1 from Streptomyces griseus: an enzyme involved in streptomycin biosynthesis., Fritsche E, Bergner A, Humm A, Piepersberg W, Huber R, Biochemistry. 1998 Dec 22;37(51):17664-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9922132 9922132]
-[[Category: Scyllo-inosamine-4-phosphate amidinotransferase]]
-[[Category: Single protein]]
 [[Category: Streptomyces griseus]]
-[[Category: Bergner, A.]]
+[[Category: Bergner A]]
-[[Category: Fritsche, E.]]
+[[Category: Fritsche E]]
-[[Category: Huber, R.]]
+[[Category: Huber R]]
-[[Category: Humm, A.]]
+[[Category: Humm A]]
-[[Category: Piepersberg, W.]]
+[[Category: Piepersberg W]]
-[[Category: Amidinotransferase]]
-[[Category: Streptomycin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:49:18 2008''