8ow0: Difference between revisions
New page: '''Unreleased structure''' The entry 8ow0 is ON HOLD Authors: Dendooven, T.D., Zhang, Z., Yang, J., McLaughlin, S., Schwabb, J., Scheres, S., Yatskevich, S., Barford, D. Description: C... |
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==Cryo-EM structure of CBF1-CCAN bound topologically to a centromeric CENP-A nucleosome== | |||
<StructureSection load='8ow0' size='340' side='right'caption='[[8ow0]], [[Resolution|resolution]] 3.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8ow0]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OW0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ow0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ow0 OCA], [https://pdbe.org/8ow0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ow0 RCSB], [https://www.ebi.ac.uk/pdbsum/8ow0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ow0 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/H4_YEAST H4_YEAST] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The point centromere of budding yeast specifies assembly of the large kinetochore complex to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner kinetochore (CCAN) complex and the microtubule-binding outer kinetochore KNL1-MIS12-NDC80 (KMN) network. The budding yeast inner kinetochore also contains the DNA binding centromere-binding factor 1 (CBF1) and CBF3 complexes. We determined the cryo-electron microscopy structure of the yeast inner kinetochore assembled onto the centromere-specific centromere protein A nucleosomes (CENP-A(Nuc)). This revealed a central CENP-A(Nuc) with extensively unwrapped DNA ends. These free DNA duplexes bind two CCAN protomers, one of which entraps DNA topologically, positioned on the centromere DNA element I (CDEI) motif by CBF1. The two CCAN protomers are linked through CBF3 forming an arch-like configuration. With a structural mechanism for how CENP-A(Nuc) can also be linked to KMN involving only CENP-QU, we present a model for inner kinetochore assembly onto a point centromere and how it organizes the outer kinetochore for chromosome attachment to the mitotic spindle. | |||
Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere.,Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D Sci Adv. 2023 Jul 28;9(30):eadg7480. doi: 10.1126/sciadv.adg7480. Epub 2023 Jul , 28. PMID:37506202<ref>PMID:37506202</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Barford | <div class="pdbe-citations 8ow0" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: Schwabb | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: | [[Category: Barford D]] | ||
[[Category: Dendooven TD]] | |||
[[Category: McLaughlin S]] | |||
[[Category: Scheres S]] | |||
[[Category: Schwabb J]] | |||
[[Category: Yang J]] | |||
[[Category: Yatskevich S]] | |||
[[Category: Zhang Z]] | |||