5gmi: Difference between revisions

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-'''Unreleased structure'''
-The entry 5gmi is ON HOLD
+==Crystal Structure of GRASP55 GRASP domain in complex with JAM-C C-terminus==
+<StructureSection load='5gmi' size='340' side='right'caption='[[5gmi]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5gmi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GMI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GMI FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.71&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gmi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gmi OCA], [https://pdbe.org/5gmi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gmi RCSB], [https://www.ebi.ac.uk/pdbsum/5gmi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gmi ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GORS2_MOUSE GORS2_MOUSE] Plays a role in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.<ref>PMID:10487747</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Spermatogenesis is a dynamic process that is regulated by adhesive interactions between germ and Sertoli cells. Germ cells express the Junctional Adhesion Molecule-C (JAM-C, encoded by Jam3), which localizes to germ/Sertoli cell contacts. JAM-C is involved in germ cell polarity and acrosome formation. Using a proteomic approach, we demonstrated that JAM-C interacted with the Golgi reassembly stacking protein of 55 kDa (GRASP55, encoded by Gorasp2) in developing germ cells. Generation and study of Gorasp2-/- mice revealed that knock-out mice suffered from spermatogenesis defects. Acrosome formation and polarized localization of JAM-C in spermatids were altered in Gorasp2-/- mice. In addition, Golgi morphology of spermatocytes was disturbed in Gorasp2-/- mice. Crystal structures of GRASP55 in complex with JAM-C or JAM-B revealed that GRASP55 interacted via PDZ-mediated interactions with JAMs and induced a conformational change in GRASP55 with respect of its free conformation. An in silico pharmacophore approach identified a chemical compound called Graspin that inhibited PDZ-mediated interactions of GRASP55 with JAMs. Treatment of mice with Graspin hampered the polarized localization of JAM-C in spermatids, induced the premature release of spermatids and affected the Golgi morphology of meiotic spermatocytes.
-Authors: Shi, N., Shi, X., Morelli, X., Betzi, S., Huang, X.
+Genetic, structural, and chemical insights into the dual function of GRASP55 in germ cell Golgi remodeling and JAM-C polarized localization during spermatogenesis.,Cartier-Michaud A, Bailly AL, Betzi S, Shi X, Lissitzky JC, Zarubica A, Serge A, Roche P, Lugari A, Hamon V, Bardin F, Derviaux C, Lembo F, Audebert S, Marchetto S, Durand B, Borg JP, Shi N, Morelli X, Aurrand-Lions M PLoS Genet. 2017 Jun 15;13(6):e1006803. doi: 10.1371/journal.pgen.1006803., eCollection 2017 Jun. PMID:28617811<ref>PMID:28617811</ref>
-Description: CRYSTAL STRUCTURE OF GRASP DOMAIN OF GRASP55 COMPLEXED WITH THE PEPTIDE
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Huang, X]]
+<div class="pdbe-citations 5gmi" style="background-color:#fffaf0;"></div>
-[[Category: Betzi, S]]
-[[Category: Morelli, X]]
+==See Also==
-[[Category: Shi, X]]
+*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
-[[Category: Shi, N]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mus musculus]]
+[[Category: Betzi S]]
+[[Category: Huang X]]
+[[Category: Morelli X]]
+[[Category: Shi N]]
+[[Category: Shi X]]