1n7l: Difference between revisions
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==Solution NMR structure of phospholamban in detergent micelles== | ==Solution NMR structure of phospholamban in detergent micelles== | ||
<StructureSection load='1n7l' size='340' side='right' caption='[[1n7l | <StructureSection load='1n7l' size='340' side='right'caption='[[1n7l]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1n7l]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1n7l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N7L FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7l OCA], [https://pdbe.org/1n7l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n7l RCSB], [https://www.ebi.ac.uk/pdbsum/1n7l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7l ProSAT]</span></td></tr> | ||
<table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/PPLA_RABIT PPLA_RABIT] Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 1n7l" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Phospholamban|Phospholamban]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Oryctolagus cuniculus]] | [[Category: Oryctolagus cuniculus]] | ||
[[Category: Mascioni | [[Category: Mascioni A]] | ||
[[Category: Thomas | [[Category: Thomas DD]] | ||
[[Category: Veglia | [[Category: Veglia G]] | ||
[[Category: Zamoon | [[Category: Zamoon J]] | ||
Latest revision as of 14:23, 2 August 2023
Solution NMR structure of phospholamban in detergent micellesSolution NMR structure of phospholamban in detergent micelles
Structural highlights
FunctionPPLA_RABIT Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. Publication Abstract from PubMedPhospholamban is an integral membrane protein that regulates the contractility of cardiac muscle by maintaining cardiomyocyte calcium homeostasis. Abnormalities in association of protein kinase A with PLB have recently been linked to human heart failure, where a single mutation is responsible for dilated cardiomyopathy. To date, a high-resolution structure of phospholamban in a lipid environment has been elusive. Here, we describe the first structure of recombinant, monomeric, biologically active phospholamban in lipid-mimicking dodecylphosphocholine micelles as determined by multidimensional NMR experiments. The overall structure of phospholamban is "L-shaped" with the hydrophobic domain approximately perpendicular to the cytoplasmic portion. This is in agreement with our previously published solid-state NMR data. In addition, there are two striking discrepancies between our structure and those reported previously for synthetic phospholamban in organic solvents: a), in our structure, the orientation of the cytoplasmic helix is consistent with the amphipathic nature of these residues; and b), within the hydrophobic helix, residues are positioned on two discrete faces of the helix as consistent with their functional roles ascribed by mutagenesis. This topology renders the two phosphorylation sites, Ser-16 and Thr-17, more accessible to kinases. NMR solution structure and topological orientation of monomeric phospholamban in dodecylphosphocholine micelles.,Zamoon J, Mascioni A, Thomas DD, Veglia G Biophys J. 2003 Oct;85(4):2589-98. PMID:14507721[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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