1cn0: Difference between revisions
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< | ==CRYSTAL STRUCTURE OF D(ACCCT)== | ||
<StructureSection load='1cn0' size='340' side='right'caption='[[1cn0]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1cn0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CN0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CN0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cn0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cn0 OCA], [https://pdbe.org/1cn0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cn0 RCSB], [https://www.ebi.ac.uk/pdbsum/1cn0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cn0 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The crystal structure of d(ACCCT), solved by molecular replacement, shows a four-stranded i-motif conformation, where two parallel duplexes intercalate with one another in opposite orientations. Each duplex is stabilized by hemi-protonated C-C+ base pairing between parallel strands, and a string of water molecules bridge the cytosine N4 atoms to phosphate O atoms. This structure of d(ACCCT) shows examples of reversed Hoogsteen and Watson-Crick base pairing in both intermolecular and intramolecular manners to stabilize the tetraplex. Noticeably, the four-stranded complex is further stabilized at one end by a three-base hydrogen-bonding network, in which two adenines and a thymine form four hydrogen bonds via a reverse Hoogsteen and an asymmetric adenine-adenine base pairing. The structure of d(ACCCT) shows a similar local structure to that found in the d(TAA) part of the crystal structure of d(TAACCC) and provides further structural evidence that these base arrangements are essential for stabilizing these novel DNA super-secondary structures. | |||
Stabilization of the i-motif by intramolecular adenine-adenine-thymine base triple in the structure of d(ACCCT).,Weil J, Min T, Yang C, Wang S, Sutherland C, Sinha N, Kang C Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):422-9. PMID:10089350<ref>PMID:10089350</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1cn0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
--> | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
== | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | |||
[[Category: Cheng Y]] | |||
[[Category: Kang C]] | |||
[[Category: Min T]] | |||
[[Category: Cheng | [[Category: Sinha N]] | ||
[[Category: Kang | [[Category: Sutherland C]] | ||
[[Category: Min | [[Category: Wang S]] | ||
[[Category: Sinha | [[Category: Weil J]] | ||
[[Category: Sutherland | |||
[[Category: Wang | |||
[[Category: Weil | |||
Latest revision as of 14:04, 2 August 2023
CRYSTAL STRUCTURE OF D(ACCCT)CRYSTAL STRUCTURE OF D(ACCCT)
Structural highlights
Publication Abstract from PubMedThe crystal structure of d(ACCCT), solved by molecular replacement, shows a four-stranded i-motif conformation, where two parallel duplexes intercalate with one another in opposite orientations. Each duplex is stabilized by hemi-protonated C-C+ base pairing between parallel strands, and a string of water molecules bridge the cytosine N4 atoms to phosphate O atoms. This structure of d(ACCCT) shows examples of reversed Hoogsteen and Watson-Crick base pairing in both intermolecular and intramolecular manners to stabilize the tetraplex. Noticeably, the four-stranded complex is further stabilized at one end by a three-base hydrogen-bonding network, in which two adenines and a thymine form four hydrogen bonds via a reverse Hoogsteen and an asymmetric adenine-adenine base pairing. The structure of d(ACCCT) shows a similar local structure to that found in the d(TAA) part of the crystal structure of d(TAACCC) and provides further structural evidence that these base arrangements are essential for stabilizing these novel DNA super-secondary structures. Stabilization of the i-motif by intramolecular adenine-adenine-thymine base triple in the structure of d(ACCCT).,Weil J, Min T, Yang C, Wang S, Sutherland C, Sinha N, Kang C Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):422-9. PMID:10089350[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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