1ahs: Difference between revisions

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[[Image:1ahs.png|left|200px]]


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==CRYSTAL STRUCTURE OF THE TOP DOMAIN OF AFRICAN HORSE SICKNESS VIRUS VP7==
The line below this paragraph, containing "STRUCTURE_1ahs", creates the "Structure Box" on the page.
<StructureSection load='1ahs' size='340' side='right'caption='[[1ahs]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1ahs]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/African_horse_sickness_virus African horse sickness virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AHS FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ahs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahs OCA], [https://pdbe.org/1ahs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ahs RCSB], [https://www.ebi.ac.uk/pdbsum/1ahs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ahs ProSAT]</span></td></tr>
{{STRUCTURE_1ahs|  PDB=1ahs  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/VP7_AHSV4 VP7_AHSV4] Major structural core protein; binds to structural protein VP3. Constitutes the surface of the AHSV core.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/1ahs_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ahs ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The baculovirus-expressed core protein VP7 of African horse sickness virus serotype 4 (AHSV-4) has been purified to homogeneity and crystallized in the presence of 2.8 M urea. The X-ray structure has been solved to a 2.3-Angstroms (1 Angstrom = 0.1 nm) resolution with an Rfactor of 19.8%. The structure of AHSV VP7 reveals that during crystallization, the two-domain protein is cleaved and only the top domain remains. A similar problem was encountered previously with bluetongue virus (BTV) VP7 (whose structure has been reported), showing that the connections between the top and the bottom domains are rather weak for these two distinct orbiviruses. The top domains of both BTV and AHSV VP7 are trimeric and structurally very similar. The electron density maps show that they both possess an extra electron density feature along their molecular threefold axes, which is most likely due to an unidentified ion. The characteristics of the molecular surface of BTV and AHSV VP7 suggest why AHSV VP7 is much less soluble than BTV VP7 and indicate the possibility of attachment to the cell via attachment of an Arg-Gly-Asp (RGD) motif in the top domain of VP7 to a cellular integrin for both of these orbiviruses.


===CRYSTAL STRUCTURE OF THE TOP DOMAIN OF AFRICAN HORSE SICKNESS VIRUS VP7===
Crystal structure of the top domain of African horse sickness virus VP7: comparisons with bluetongue virus VP7.,Basak AK, Gouet P, Grimes J, Roy P, Stuart D J Virol. 1996 Jun;70(6):3797-806. PMID:8648715<ref>PMID:8648715</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_8648715}}, adds the Publication Abstract to the page
<div class="pdbe-citations 1ahs" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 8648715 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_8648715}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: African horse sickness virus]]
1AHS is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/African_horsesickness_virus African horsesickness virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHS OCA].
[[Category: Large Structures]]
 
[[Category: Gouet P]]
==Reference==
[[Category: Stuart D]]
<ref group="xtra">PMID:8648715</ref><references group="xtra"/>
[[Category: African horsesickness virus]]
[[Category: Gouet, P.]]
[[Category: Stuart, D.]]
[[Category: Core protein]]
[[Category: Glycoprotein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:10:44 2009''

Latest revision as of 13:52, 2 August 2023

CRYSTAL STRUCTURE OF THE TOP DOMAIN OF AFRICAN HORSE SICKNESS VIRUS VP7CRYSTAL STRUCTURE OF THE TOP DOMAIN OF AFRICAN HORSE SICKNESS VIRUS VP7

Structural highlights

1ahs is a 3 chain structure with sequence from African horse sickness virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VP7_AHSV4 Major structural core protein; binds to structural protein VP3. Constitutes the surface of the AHSV core.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The baculovirus-expressed core protein VP7 of African horse sickness virus serotype 4 (AHSV-4) has been purified to homogeneity and crystallized in the presence of 2.8 M urea. The X-ray structure has been solved to a 2.3-Angstroms (1 Angstrom = 0.1 nm) resolution with an Rfactor of 19.8%. The structure of AHSV VP7 reveals that during crystallization, the two-domain protein is cleaved and only the top domain remains. A similar problem was encountered previously with bluetongue virus (BTV) VP7 (whose structure has been reported), showing that the connections between the top and the bottom domains are rather weak for these two distinct orbiviruses. The top domains of both BTV and AHSV VP7 are trimeric and structurally very similar. The electron density maps show that they both possess an extra electron density feature along their molecular threefold axes, which is most likely due to an unidentified ion. The characteristics of the molecular surface of BTV and AHSV VP7 suggest why AHSV VP7 is much less soluble than BTV VP7 and indicate the possibility of attachment to the cell via attachment of an Arg-Gly-Asp (RGD) motif in the top domain of VP7 to a cellular integrin for both of these orbiviruses.

Crystal structure of the top domain of African horse sickness virus VP7: comparisons with bluetongue virus VP7.,Basak AK, Gouet P, Grimes J, Roy P, Stuart D J Virol. 1996 Jun;70(6):3797-806. PMID:8648715[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Basak AK, Gouet P, Grimes J, Roy P, Stuart D. Crystal structure of the top domain of African horse sickness virus VP7: comparisons with bluetongue virus VP7. J Virol. 1996 Jun;70(6):3797-806. PMID:8648715

1ahs, resolution 2.30Å

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