5frd: Difference between revisions
New page: '''Unreleased structure''' The entry 5frd is ON HOLD Authors: Sayer, C., Finnigan, W., Isupov, M.N., Levisson, M., Kengen, S.W.M., van der Oost, J., Harmer, N., Littlechild, J.A. Descr... |
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The | ==Structure of a thermophilic esterase== | ||
<StructureSection load='5frd' size='340' side='right'caption='[[5frd]], [[Resolution|resolution]] 1.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5frd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FRD FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5frd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5frd OCA], [https://pdbe.org/5frd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5frd RCSB], [https://www.ebi.ac.uk/pdbsum/5frd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5frd ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/O28735_ARCFU O28735_ARCFU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A new carboxyl esterase, AF-Est2, from the hyperthermophilic archaeon Archaeoglobus fulgidus has been cloned, over-expressed in Escherichia coli and biochemically and structurally characterized. The enzyme has high activity towards short- to medium-chain p-nitrophenyl carboxylic esters with optimal activity towards the valerate ester. The AF-Est2 has good solvent and pH stability and is very thermostable, showing no loss of activity after incubation for 30 min at 80 degrees C. The 1.4 A resolution crystal structure of AF-Est2 reveals Coenzyme A (CoA) bound in the vicinity of the active site. Despite the presence of CoA bound to the AF-Est2 this enzyme has no CoA thioesterase activity. The pantetheine group of CoA partially obstructs the active site alcohol pocket suggesting that this ligand has a role in regulation of the enzyme activity. A comparison with closely related alpha/beta hydrolase fold enzyme structures shows that the AF-Est2 has unique structural features that allow CoA binding. A comparison of the structure of AF-Est2 with the human carboxyl esterase 1, which has CoA thioesterase activity, reveals that CoA is bound to different parts of the core domain in these two enzymes and approaches the active site from opposite directions. | |||
Structural and biochemical characterisation of Archaeoglobus fulgidus esterase reveals a bound CoA molecule in the vicinity of the active site.,Sayer C, Finnigan W, Isupov MN, Levisson M, Kengen SW, van der Oost J, Harmer NJ, Littlechild JA Sci Rep. 2016 May 10;6:25542. doi: 10.1038/srep25542. PMID:27160974<ref>PMID:27160974</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5frd" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Carboxylesterase 3D structures|Carboxylesterase 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Littlechild | __TOC__ | ||
[[Category: Sayer | </StructureSection> | ||
[[Category: Archaeoglobus fulgidus]] | |||
[[Category: Large Structures]] | |||
[[Category: Finnigan W]] | |||
[[Category: Harmer N]] | |||
[[Category: Isupov MN]] | |||
[[Category: Kengen SWM]] | |||
[[Category: Levisson M]] | |||
[[Category: Littlechild JA]] | |||
[[Category: Sayer C]] | |||
[[Category: Van der Oost J]] | |||