3cww: Difference between revisions

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-[[Image:3cww.png|left|200px]]
-{{STRUCTURE_3cww|  PDB=3cww  |  SCENE=  }}
+==Crystal Structure of IDE-bradykinin complex==
+<StructureSection load='3cww' size='340' side='right'caption='[[3cww]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3cww]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CWW FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cww OCA], [https://pdbe.org/3cww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cww RCSB], [https://www.ebi.ac.uk/pdbsum/3cww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cww ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IDE_HUMAN IDE_HUMAN] Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.<ref>PMID:10684867</ref> <ref>PMID:17613531</ref> <ref>PMID:18986166</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Insulin degrading enzyme (IDE) utilizes a large catalytic chamber to selectively bind and degrade peptide substrates such as insulin and amyloid beta (Abeta). Tight interactions with substrates occur at an exosite located approximately 30 A away from the catalytic center that anchors the N-terminus of substrates to facilitate binding and subsequent cleavages at the catalytic site. However, IDE also degrades peptide substrates that are too short to occupy both the catalytic site and the exosite simultaneously. Here, we use kinins as a model system to address the kinetics and regulation of human IDE with short peptides. IDE specifically degrades bradykinin and kallidin at the Pro/Phe site. A 1.9 A crystal structure of bradykinin-bound IDE reveals the binding of bradykinin to the exosite and not to the catalytic site. In agreement with observed high K m values, this suggests low affinity of bradykinin for IDE. This structure also provides the molecular basis on how the binding of short peptides at the exosite could regulate substrate recognition. We also found that human IDE is potently inhibited by physiologically relevant concentrations of S-nitrosylation and oxidation agents. Cysteine-directed modifications play a key role, since an IDE mutant devoid of all 13 cysteines is insensitive to the inhibition by S-nitrosoglutathione, hydrogen peroxide, or N-ethylmaleimide. Specifically, cysteine 819 of human IDE is located inside the catalytic chamber pointing toward an extended hydrophobic pocket and is critical for the inactivation. Thiol-directed modification of this residue likely causes local structural perturbation to reduce substrate binding and catalysis.
-===Crystal Structure of IDE-bradykinin complex===
+Molecular Bases for the Recognition of Short Peptide Substrates and Cysteine-Directed Modifications of Human Insulin-Degrading Enzyme.,Malito E, Ralat LA, Manolopoulou M, Tsay JL, Wadlington NL, Tang WJ Biochemistry. 2008 Nov 6. PMID:18986166<ref>PMID:18986166</ref>
-{{ABSTRACT_PUBMED_18986166}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 3cww" style="background-color:#fffaf0;"></div>
-[[3cww]] is a 4 chain structure of [[Insulin-Degrading Enzyme]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CWW OCA].
 ==See Also==
-*[[Insulin-Degrading Enzyme|Insulin-Degrading Enzyme]]
+*[[Insulin-degrading enzyme 3D structures|Insulin-degrading enzyme 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:018986166</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Insulysin]]
+[[Category: Large Structures]]
-[[Category: Malito, E.]]
+[[Category: Malito E]]
-[[Category: Tang, W J.]]
+[[Category: Tang WJ]]
-[[Category: A-beta degrading enzyme]]
-[[Category: Bradykinin]]
-[[Category: Criptidase]]
-[[Category: Hydrolase]]
-[[Category: Kinin]]