5fet: Difference between revisions
New page: '''Unreleased structure''' The entry 5fet is ON HOLD Authors: Wernimont, A.K., Tempel, W., Walker, J.R, He, H., Seitova, A., Hills, T., Neculai, A.M., Baker, D.A, Flueck, C., Kettleboro... |
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==Crystal Structure of PVX_084705 in presence of Compound 2== | |||
<StructureSection load='5fet' size='340' side='right'caption='[[5fet]], [[Resolution|resolution]] 3.07Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5fet]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_vivax_Sal-1 Plasmodium vivax Sal-1]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5f0d 5f0d] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4oxj 4oxj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FET FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.07Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1TR:4-[7-[(DIMETHYLAMINO)METHYL]-2-(4-FLUOROPHENYL)IMIDAZO[1,2-A]PYRIDIN-3-YL]PYRIMIDIN-2-AMINE'>1TR</scene></td></tr> | ||
[[Category: | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fet OCA], [https://pdbe.org/5fet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fet RCSB], [https://www.ebi.ac.uk/pdbsum/5fet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fet ProSAT]</span></td></tr> | ||
[[Category: | </table> | ||
[[Category: | == Function == | ||
[[Category: | [https://www.uniprot.org/uniprot/KGP_PLAVS KGP_PLAVS] Serine/threonine protein kinase which acts as a downstream effector of the second messenger cGMP. Controls the release of Ca(2+) from intracellular stores by regulating phosphoinositide biosynthesis. Ca(2+) signals are essential for merozoite and sporozoite invasion and egress from host hepatocytes and erythrocytes, and, in the mosquito vector, for gametocyte activation, and ookinete and sporozoite motility (By similarity). During the host liver stage, regulates the initial invasion of host hepatocytes by sporozoites by regulating sporozoite motility and microneme exocytosis. Following parasite development in the hepatocytes, required for the release of merosomes, a vesicle containing the mature merozoites (By similarity). During the asexual blood stage, required for the progression from schizont to the ring stage following merozoite invasion of host erythrocytes and for merozoite egress. Regulates merozoite egress by promoting the release of exonemes and micronemes which contain proteins essential for egress. Phosphorylates CDPK1 predominantly at the late schizont stage; phosphorylation at 'Ser-64' regulates CDPK1 protein-protein interaction and phosphorylation at 'Thr-231' may regulate CDPK1 kinase activity. In the mosquito vector, required for the initiation of gametogenesis induced by xanthurenic acid, specifically the gametocyte differentiation from the crescent-shaped form to the spherical form (By similarity). Required for the gliding motility of ookinetes to reach and penetrate the midgut epithelium by promoting Ca(2+)-mediated activation of CDPK1 and CDPK4. Also required for microneme secretion in ookinete by promoting Ca(2+)-mediated activation of CDPK3 (By similarity).[UniProtKB:A0A509AKL0][UniProtKB:Q8I719] | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Plasmodium vivax Sal-1]] | ||
[[Category: | [[Category: Arrowsmith CH]] | ||
[[Category: | [[Category: Baker DA]] | ||
[[Category: Kettleborough | [[Category: Bountra C]] | ||
[[Category: | [[Category: Edwards AM]] | ||
[[Category: | [[Category: El Bakkouri M]] | ||
[[Category: | [[Category: Flueck C]] | ||
[[Category: | [[Category: He H]] | ||
[[Category: Hills T]] | |||
[[Category: Hui R]] | |||
[[Category: Hutchinson A]] | |||
[[Category: Kettleborough CA]] | |||
[[Category: Neculai AM]] | |||
[[Category: Seitova A]] | |||
[[Category: Tempel W]] | |||
[[Category: Walker JR]] | |||
[[Category: Wernimont AK]] | |||
Latest revision as of 09:48, 19 July 2023
Crystal Structure of PVX_084705 in presence of Compound 2Crystal Structure of PVX_084705 in presence of Compound 2
Structural highlights
FunctionKGP_PLAVS Serine/threonine protein kinase which acts as a downstream effector of the second messenger cGMP. Controls the release of Ca(2+) from intracellular stores by regulating phosphoinositide biosynthesis. Ca(2+) signals are essential for merozoite and sporozoite invasion and egress from host hepatocytes and erythrocytes, and, in the mosquito vector, for gametocyte activation, and ookinete and sporozoite motility (By similarity). During the host liver stage, regulates the initial invasion of host hepatocytes by sporozoites by regulating sporozoite motility and microneme exocytosis. Following parasite development in the hepatocytes, required for the release of merosomes, a vesicle containing the mature merozoites (By similarity). During the asexual blood stage, required for the progression from schizont to the ring stage following merozoite invasion of host erythrocytes and for merozoite egress. Regulates merozoite egress by promoting the release of exonemes and micronemes which contain proteins essential for egress. Phosphorylates CDPK1 predominantly at the late schizont stage; phosphorylation at 'Ser-64' regulates CDPK1 protein-protein interaction and phosphorylation at 'Thr-231' may regulate CDPK1 kinase activity. In the mosquito vector, required for the initiation of gametogenesis induced by xanthurenic acid, specifically the gametocyte differentiation from the crescent-shaped form to the spherical form (By similarity). Required for the gliding motility of ookinetes to reach and penetrate the midgut epithelium by promoting Ca(2+)-mediated activation of CDPK1 and CDPK4. Also required for microneme secretion in ookinete by promoting Ca(2+)-mediated activation of CDPK3 (By similarity).[UniProtKB:A0A509AKL0][UniProtKB:Q8I719] |
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