5eyw: Difference between revisions

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-'''Unreleased structure'''
-The entry 5eyw is ON HOLD
+==Crystal structure of Litopenaeus vannamei triosephosphate isomerase complexed with 2-Phosphoglycolic acid==
+<StructureSection load='5eyw' size='340' side='right'caption='[[5eyw]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5eyw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Penaeus_vannamei Penaeus vannamei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EYW FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGA:2-PHOSPHOGLYCOLIC+ACID'>PGA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eyw OCA], [https://pdbe.org/5eyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eyw RCSB], [https://www.ebi.ac.uk/pdbsum/5eyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eyw ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/K0E682_PENVA K0E682_PENVA]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Triosephosphate isomerase (TIM; EC 5.3.1.1) is a key enzyme involved in glycolysis and gluconeogenesis. Glycolysis is one of the most regulated metabolic pathways, however little is known about the structural mechanisms for its regulation in non-model organisms, like crustaceans. To understand the structure and function of this enzyme in invertebrates, we obtained the crystal structure of triosephosphate isomerase from the marine Pacific whiteleg shrimp (Litopenaeus vannamei, LvTIM) in complex with its inhibitor 2-phosphogyceric acid (2-PG) at 1.7A resolution. LvTIM assembles as a homodimer with residues 166-176 covering the active site and residue Glu166 interacting with the inhibitor. We found that LvTIM is the least stable TIM characterized to date, with the lowest range of melting temperatures, and with the lowest activation enthalpy associated with the thermal unfolding process reported. In TIMs dimer stabilization is maintained by an interaction of loop 3 by a set of hydrophobic contacts between subunits. Within these contacts, the side chain of a hydrophobic residue of one subunit fits into a cavity created by a set of hydrophobic residues in the neighboring subunit, via a "ball and socket" interaction. LvTIM presents a Cys47 at the "ball" inter-subunit contact indicating that the character of this residue is responsible for the decrease in dimer stability. Mutational studies show that this residue plays a role in dimer stability but is not a solely determinant for dimer formation.
-Authors: Carrasco-Miranda, J.S., Lopez-Zavala.A.A., Brieba, L.G, Rudino-Pinera, E., Sotelo-Mundo, R.R.
+Structural insights from a novel invertebrate triosephosphate isomerase from Litopenaeus vannamei.,Lopez-Zavala AA, Carrasco-Miranda JS, Ramirez-Aguirre CD, Lopez-Hidalgo M, Benitez-Cardoza CG, Ochoa-Leyva A, Cardona-Felix CS, Diaz-Quezada C, Rudino-Pinera E, Sotelo-Mundo RR, Brieba LG Biochim Biophys Acta. 2016 Sep 7;1864(12):1696-1706. doi:, 10.1016/j.bbapap.2016.09.002. PMID:27614148<ref>PMID:27614148</ref>
-Description: Crystal structure of Litopenaeus vannamei triosephosphate isomerase complexed with 2-Phosphoglycolic acid
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Carrasco-Miranda, J.S]]
+<div class="pdbe-citations 5eyw" style="background-color:#fffaf0;"></div>
-[[Category: Lopez-Zavala.A.A]]
+== References ==
-[[Category: Brieba, L.G, Rudino-Pinera, E]]
+<references/>
-[[Category: Sotelo-Mundo, R.R]]
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Penaeus vannamei]]
+[[Category: Brieba LG]]
+[[Category: Carrasco-Miranda JS]]
+[[Category: Lopez-Zavala AA]]
+[[Category: Rudino-Pinera E]]
+[[Category: Sotelo-Mundo RR]]