5es3: Difference between revisions

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-'''Unreleased structure'''
-The entry 5es3 is ON HOLD  until Paper Publication
+==Co-crystal structure of LDH liganded with oxamate==
+<StructureSection load='5es3' size='340' side='right'caption='[[5es3]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5es3]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ES3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ES3 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.29&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OXM:OXAMIC+ACID'>OXM</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5es3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5es3 OCA], [https://pdbe.org/5es3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5es3 RCSB], [https://www.ebi.ac.uk/pdbsum/5es3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5es3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LDHA_RAT LDHA_RAT]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We developed an efficient, automated 2-step purification protocol for the production of milligram quantities of untagged recombinant rat lactate dehydrogenase A (rLDHA) from E. coli, using the AKTAxpress chromatography system. Cation exchange followed by size exclusion results in average final purity in excess of 93% and yields ~ 14 milligrams per 50 ml of original cell culture in EnPresso B media, in under 8 hrs, including all primary sample processing and column equilibration steps. The protein is highly active and coherent biophysically and a viable alternative to the more problematic human homolog for structural and ligand-binding studies; an apo structure of untagged rLDHA was solved to a resolution 2.29 A (PDB ID 5ES3). Our automated methodology uses generic commercially available pre-packed columns and simple buffers, and represents a robust standard method for the production of milligram amounts of untagged rLDHA, facilitating a novel fragment screening approach for new inhibitors.
-Authors: Nowicki, M.W., Wear, M.A., McNae, I.W., Blackburn, E.A.
+A Streamlined, Automated Protocol for the Production of Milligram Quantities of Untagged Recombinant Rat Lactate Dehydrogenase A Using AKTAxpressTM.,Nowicki MW, Blackburn EA, McNae IW, Wear MA PLoS One. 2015 Dec 30;10(12):e0146164. doi: 10.1371/journal.pone.0146164., eCollection 2015. PMID:26717415<ref>PMID:26717415</ref>
-Description: Co-crystal structure of LDH liganded with oxamate
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Nowicki, M.W]]
+<div class="pdbe-citations 5es3" style="background-color:#fffaf0;"></div>
-[[Category: Blackburn, E.A]]
-[[Category: Mcnae, I.W]]
+==See Also==
-[[Category: Wear, M.A]]
+*[[Lactate dehydrogenase 3D structures|Lactate dehydrogenase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Rattus norvegicus]]
+[[Category: Blackburn EA]]
+[[Category: McNae IW]]
+[[Category: Nowicki MW]]
+[[Category: Wear MA]]