5eqw: Difference between revisions

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-'''Unreleased structure'''
-The entry 5eqw is ON HOLD  until Nov 13 2017
+==Structure of the major structural protein D135 of Acidianus tailed spindle virus (ATSV)==
+<StructureSection load='5eqw' size='340' side='right'caption='[[5eqw]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5eqw]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidianus_tailed_spindle_virus Acidianus tailed spindle virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EQW FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.679&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eqw OCA], [https://pdbe.org/5eqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eqw RCSB], [https://www.ebi.ac.uk/pdbsum/5eqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eqw ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A125SJ78_9VIRU A0A125SJ78_9VIRU]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The spindle-shaped virion morphology is common among archaeal viruses, where it is a defining characteristic of many viral families. However, structural heterogeneity intrinsic to spindle-shaped viruses has seriously hindered efforts to elucidate the molecular architecture of these lemon-shaped capsids. We have utilized a combination of cryo-electron microscopy and X-ray crystallography to study Acidianus tailed spindle virus (ATSV). These studies reveal the architectural principles that underlie assembly of a spindle-shaped virus. Cryo-electron tomography shows a smooth transition from the spindle-shaped capsid into the tubular-shaped tail and allows low-resolution structural modeling of individual virions. Remarkably, higher-dose 2D micrographs reveal a helical surface lattice in the spindle-shaped capsid. Consistent with this, crystallographic studies of the major capsid protein reveal a decorated four-helix bundle that packs within the crystal to form a four-start helical assembly with structural similarity to the tube-shaped tail structure of ATSV and other tailed, spindle-shaped viruses. Combined, this suggests that the spindle-shaped morphology of the ATSV capsid is formed by a multistart helical assembly with a smoothly varying radius and allows construction of a pseudoatomic model for the lemon-shaped capsid that extends into a tubular tail. The potential advantages that this novel architecture conveys to the life cycle of spindle-shaped viruses, including a role in DNA ejection, are discussed.
-Authors: Hochstein, R.A., Lintner, N.G., Young, M.J., Lawrence, C.M.
+Structural studies of Acidianus tailed spindle virus reveal a structural paradigm used in the assembly of spindle-shaped viruses.,Hochstein R, Bollschweiler D, Dharmavaram S, Lintner NG, Plitzko JM, Bruinsma R, Engelhardt H, Young MJ, Klug WS, Lawrence CM Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2120-2125. doi:, 10.1073/pnas.1719180115. Epub 2018 Feb 12. PMID:29440399<ref>PMID:29440399</ref>
-Description: Structure of the major structural protein D135 of Acidianus tailed spindle virus (ATSV)
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Lawrence, C.M]]
+<div class="pdbe-citations 5eqw" style="background-color:#fffaf0;"></div>
-[[Category: Lintner, N.G]]
+== References ==
-[[Category: Young, M.J]]
+<references/>
-[[Category: Hochstein, R.A]]
+__TOC__
+</StructureSection>
+[[Category: Acidianus tailed spindle virus]]
+[[Category: Large Structures]]
+[[Category: Hochstein RA]]
+[[Category: Lawrence CM]]
+[[Category: Lintner NG]]
+[[Category: Young MJ]]