5emy: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5emy is ON HOLD  until Paper Publication
+==Human Pancreatic Alpha-Amylase in complex with the mechanism based inactivator glucosyl epi-cyclophellitol==
+<StructureSection load='5emy' size='340' side='right'caption='[[5emy]], [[Resolution|resolution]] 1.23&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5emy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EMY FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.231&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5QP:(2~{R},3~{S},4~{S},5~{R},6~{S})-2-(HYDROXYMETHYL)-6-[(1~{R},2~{S},3~{R},5~{S},6~{R})-2-(HYDROXYMETHYL)-3,5,6-TRIS(OXIDANYL)CYCLOHEXYL]OXY-OXANE-3,4,5-TRIOL'>5QP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5emy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5emy OCA], [https://pdbe.org/5emy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5emy RCSB], [https://www.ebi.ac.uk/pdbsum/5emy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5emy ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AMYP_HUMAN AMYP_HUMAN]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+As part of a search for selective, mechanism-based covalent inhibitors of human pancreatic alpha-amylase we describe the chemoenzymatic synthesis of the disaccharide analog alpha-glucosyl epi-cyclophellitol, demonstrate its stoichiometric reaction with human pancreatic alpha-amylase and evaluate the time dependence of its inhibition. X-ray crystallographic analysis of the covalent derivative so formed confirms its reaction at the active site with formation of a covalent bond to the catalytic nucleophile D197. The structure illuminates the interactions with the active site and confirms OH4' on the nonreducing end sugar as a good site for attachment of fluorescent tags in generating probes for localization and quantitation of amylase in vivo.
-Authors: Caner, S., Brayer, G.D.
+Glucosyl epi-cyclophellitol allows mechanism-based inactivation and structural analysis of human pancreatic alpha-amylase.,Caner S, Zhang X, Jiang J, Chen HM, Nguyen NT, Overkleeft H, Brayer GD, Withers SG FEBS Lett. 2016 Apr;590(8):1143-51. doi: 10.1002/1873-3468.12143. Epub 2016 Apr, 3. PMID:27000970<ref>PMID:27000970</ref>
-Description: Human Pancreatic Alpha-Amylase in complex with the mechanism based inactivator glucosyl epi-cyclophellitol
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Caner, S]]
+<div class="pdbe-citations 5emy" style="background-color:#fffaf0;"></div>
-[[Category: Brayer, G.D]]
+==See Also==
+*[[Amylase 3D structures|Amylase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Brayer GD]]
+[[Category: Caner S]]