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==Crystal structure of the PKI NES-CRM1-RanGTP nuclear export complex== | ==Crystal structure of the PKI NES-CRM1-RanGTP nuclear export complex== | ||
<StructureSection load='3nby' size='340' side='right' caption='[[3nby]], [[Resolution|resolution]] 3.42Å' scene=''> | <StructureSection load='3nby' size='340' side='right'caption='[[3nby]], [[Resolution|resolution]] 3.42Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3nby]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3nby]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NBY FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.42Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nby OCA], [https://pdbe.org/3nby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nby RCSB], [https://www.ebi.ac.uk/pdbsum/3nby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nby ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/SPN1_HUMAN SPN1_HUMAN] Functions as an U snRNP-specific nuclear import adapter. Involved in the trimethylguanosine (m3G)-cap-dependent nuclear import of U snRNPs. Binds specifically to the terminal m3G-cap U snRNAs.<ref>PMID:9670026</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 17: | Line 18: | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3nby" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Exportin|Exportin]] | *[[Exportin 3D structures|Exportin 3D structures]] | ||
*[[GTP-binding protein|GTP-binding protein]] | *[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 26: | Line 28: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Corsini | [[Category: Corsini L]] | ||
[[Category: Deichsel | [[Category: Deichsel D]] | ||
[[Category: Ficner | [[Category: Ficner R]] | ||
[[Category: Gorlich | [[Category: Gorlich D]] | ||
[[Category: Guttler | [[Category: Guttler T]] | ||
[[Category: Madl | [[Category: Madl T]] | ||
[[Category: Monecke | [[Category: Monecke T]] | ||
[[Category: Neumann | [[Category: Neumann P]] | ||
[[Category: Sattler | [[Category: Sattler M]] | ||
Latest revision as of 10:57, 12 July 2023
Crystal structure of the PKI NES-CRM1-RanGTP nuclear export complexCrystal structure of the PKI NES-CRM1-RanGTP nuclear export complex
Structural highlights
FunctionSPN1_HUMAN Functions as an U snRNP-specific nuclear import adapter. Involved in the trimethylguanosine (m3G)-cap-dependent nuclear import of U snRNPs. Binds specifically to the terminal m3G-cap U snRNAs.[1] Publication Abstract from PubMedClassic nuclear export signals (NESs) confer CRM1-dependent nuclear export. Here we present crystal structures of the RanGTP-CRM1 complex alone and bound to the prototypic PKI or HIV-1 Rev NESs. These NESs differ markedly in the spacing of their key hydrophobic (Phi) residues, yet CRM1 recognizes them with the same rigid set of five Phi pockets. The different Phi spacings are compensated for by different conformations of the bound NESs: in the case of PKI, an alpha-helical conformation, and in the case of Rev, an extended conformation with a critical proline docking into a Phi pocket. NMR analyses of CRM1-bound and CRM1-free PKI NES suggest that CRM1 selects NES conformers that pre-exist in solution. Our data lead to a new structure-based NES consensus, and explain why NESs differ in their affinities for CRM1 and why supraphysiological NESs bind the exportin so tightly. NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1.,Guttler T, Madl T, Neumann P, Deichsel D, Corsini L, Monecke T, Ficner R, Sattler M, Gorlich D Nat Struct Mol Biol. 2010 Oct 24. PMID:20972448[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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