8h1m: Difference between revisions

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-'''Unreleased structure'''
-The entry 8h1m is ON HOLD  until Paper Publication
+==Crystal structure of glucose-2-epimerase mutant_D254A from Runella slithyformis Runsl_4512==
+<StructureSection load='8h1m' size='340' side='right'caption='[[8h1m]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8h1m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Runella_slithyformis Runella slithyformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H1M FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h1m OCA], [https://pdbe.org/8h1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h1m RCSB], [https://www.ebi.ac.uk/pdbsum/8h1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h1m ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A7U4E834_RUNSL A0A7U4E834_RUNSL]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Mannose 2-epimerase (ME), a member of the acylglucosamine 2-epimerase (AGE) superfamily that catalyzes epimerization of D-mannose and D-glucose, has recently been characterized to have potential for D-mannose production. However, the substrate-recognition and catalytic mechanism of ME remains unknown. In this study, structures of Runella slithyformis ME (RsME) and its D254A mutant [RsME(D254A)] were determined in their apo forms and as intermediate-analog complexes [RsME-D-glucitol and RsME(D254A)-D-glucitol]. RsME possesses the (alpha/alpha)(6)-barrel of the AGE superfamily members but has a unique pocket-covering long loop (loop(alpha7-alpha8)). The RsME-D-glucitol structure showed that loop(alpha7-alpha8) moves towards D-glucitol and closes the active pocket. Trp251 and Asp254 in loop(alpha7-alpha8) are only conserved in MEs and interact with D-glucitol. Kinetic analyses of the mutants confirmed the importance of these residues for RsME activity. Moreover, the structures of RsME(D254A) and RsME(D254A)-D-glucitol revealed that Asp254 is vital for binding the ligand in a correct conformation and for active-pocket closure. Docking calculations and structural comparison with other 2-epimerases show that the longer loop(alpha7-alpha8) in RsME causes steric hindrance upon binding to disaccharides. A detailed substrate-recognition and catalytic mechanism for monosaccharide-specific epimerization in RsME has been proposed.
-Authors: Wang, H., Sun, X.M., Saburi, W., Yu, J., Yao, M.
+Structural insights into the substrate specificity and activity of a novel mannose 2-epimerase from Runella slithyformis.,Wang H, Sun X, Saburi W, Hashiguchi S, Yu J, Ose T, Mori H, Yao M Acta Crystallogr D Struct Biol. 2023 Jul 1;79(Pt 7):585-595. doi: , 10.1107/S205979832300390X. Epub 2023 Jun 14. PMID:37314406<ref>PMID:37314406</ref>
-Description: Crystal structure of glucose-2-epimerase mutant_D254A from Runella slithyformis Runsl_4512
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Sun, X.M]]
+<div class="pdbe-citations 8h1m" style="background-color:#fffaf0;"></div>
-[[Category: Saburi, W]]
+== References ==
-[[Category: Yao, M]]
+<references/>
-[[Category: Wang, H]]
+__TOC__
-[[Category: Yu, J]]
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Runella slithyformis]]
+[[Category: Saburi W]]
+[[Category: Sun XM]]
+[[Category: Wang H]]
+[[Category: Yao M]]
+[[Category: Yu J]]