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| == Lysozyme ==
| | <StructureSection load='3wja' size='340' side='right' caption='The best structure for NADP-dependent malic enzyme shown: [[3wja]]' scene=''> |
| <StructureSection load='1swy' size='340' side='right' caption='Caption for this structure' scene=''> | | Best example is PDB entry [[3wja]] and is shown in the viewer.<br> |
| Best example is 1swy<br> | | ==Catalytic Activity == |
| Molecule Lysozyme, also known as Lysozyme, CP-7 lysin, Muramidase, Endolysin, 1,4-beta-N-acetylmuramidase, Lysis protein, CP-1 lysin, Lysozyme murein hydrolase, Protein gp17, Transglycosylase, Hevamine-A, Chitinase and L-alanyl-D-glutamate peptidase.
| | Oxaloacetate = pyruvate + CO(2).Data source: Uniprot [http://www.uniprot.org/uniprot/P48163 P48163]<br> |
| == Function == | | Or the decarboxylation of malate to pyruvate<ref>PMID:8187880</ref>. |
| lysozyme activity<br>
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| catalytic activity<br>
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| hydrolase activity<br>
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| hydrolase activity, acting on glycosyl bonds<br>
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| carbon-oxygen lyase activity, acting on polysaccharides<br>
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| lyase activity<br>
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| chitinase activity<br>
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| hydrolase activity, hydrolyzing O-glycosyl compounds<br>
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| sequence-specific DNA binding<br>
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| == Disease ==
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| == Relevance ==
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| == Structural highlights ==
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| == Biological process == | | == Biological process == |
| Is involved in the following biological processes:<br> | | Is involved in the following biological processes:<br> |
| cell wall macromolecule catabolic process<br>
| | malate metabolic process<br> |
| peptidoglycan catabolic process<br>
| | oxidation-reduction process<br> |
| metabolic process<br>
| | response to carbohydrate<br> |
| defense response to bacterium<br>
| | response to hormone<br> |
| cytolysis<br>
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| viral release from host cell<br>
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| carbohydrate metabolic process<br> | | carbohydrate metabolic process<br> |
| chitin catabolic process<br>
| | protein tetramerization<br> |
| polysaccharide catabolic process<br>
| | regulation of NADP metabolic process<br> |
| cytolysis by virus of host cell<br>
| | NADP biosynthetic process<br> |
| cell wall organization<br>
| | small molecule metabolic process<br> |
| | cellular lipid metabolic process<br> |
| == In structures == | | == In structures == |
| Lysozyme is found in 595 PDB entries<br>
| | NADP-dependent malic enzyme is found in 3 PDB entries<br> |
| *<b>Eukaryota</b> (16 PDB entries): <br> | | *<b>Eukaryota</b> (3 PDB entries): <br> |
| **<b>Homo sapiens</b> (1 PDB entries): <br> | | **<b>Homo sapiens</b> (2 PDB entries): <br> |
| ***[[ 4uis]] <div class="pdb-prints 4uis"></div> | | ***[[ 3wja]] <div class="pdb-prints 3wja"></div> |
| ***Title: The cryoEM structure of human gamma-Secretase complex | | ***Title: The crystal structure of human cytosolic NADP(+)-dependent malic enzyme in apo form |
| ***Experimental EM map [http://pdbe.org/emd-2974 emd-2974].
| | ***2.548 A resolution |
| ***4.4 A resolution
| | ***[http://www.ebi.ac.uk/pdbe/entry/search/index?organism_scientific_name:%22Homo%20sapiens%22&all_molecule_names:%22NADP-dependent%20malic%20enzyme%22&!chimera:y Search the PDB for NADP-dependent malic enzyme from Homo sapiens] |
| ***Other macromolecules also in this entry: GAMMA-SECRETASE, Nicastrin, Presenilin-1 NTF subunit, .
| | **<b>Columba livia</b> (1 PDB entries): <br> |
| <br/>
| | ***[[ 1gq2]] <div class="pdb-prints 1gq2"></div> |
| **<b>Hevea brasiliensis</b> (7 PDB entries): <br>
| | ***Title: MALIC ENZYME FROM PIGEON LIVER |
| ***[[1kqy]] <div class="pdb-prints 1kqy"></div>
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| ***Title: Hevamine Mutant D125A/E127A/Y183F in Complex with Penta-NAG
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| ***1.92 A resolution | |
| | |
| ***[http://www.ebi.ac.uk/pdbe/entry/search/index?organism_scientific:%34Hevea%20brasiliensis%34&molecule_name:%34Lysozyme%34 Search for other PDB entries] <br/> | |
| **<b>Meretrix lusoria</b> (3 PDB entries): <br>
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| ***[[4pj2]] <div class="pdb-prints 4pj2"></div>
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| ***Title: Crystal structure of Aeromonas hydrophila PliI in complex with Meretrix lusoria lysozyme
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| ***1.24 A resolution
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| ***Other macromolecules also in this entry: Putative exported protein, .
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| ***[http://www.ebi.ac.uk/pdbe/entry/search/index?organism_scientific:%34Meretrix%20lusoria%34&molecule_name:%34Lysozyme%34 Search for other PDB entries] <br/>
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| **<b>Antheraea mylitta</b> (1 PDB entries): <br>
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| ***[[1iiz]] <div class="pdb-prints 1iiz"></div>
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| ***Title: Crystal Structure of the Induced Antibacterial Protein from Tasar Silkworm, Antheraea mylitta
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| ***2.4 A resolution
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| <br/>
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| **<b>Ruditapes philippinarum</b> (1 PDB entries): <br>
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| ***[[2dqa]] <div class="pdb-prints 2dqa"></div>
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| ***Title: Crystal Structure of Tapes japonica Lysozyme
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| ***1.6 A resolution
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| <br/>
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| **<b>Mus musculus</b> (1 PDB entries): <br> | |
| ***[[1iak]] <div class="pdb-prints 1iak"></div> | |
| ***Title: HISTOCOMPATIBILITY ANTIGEN I-AK
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| ***1.9 A resolution
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| ***Other macromolecules also in this entry: H-2 class II histocompatibility antigen, A-K beta chain, H-2 class II histocompatibility antigen, A-K alpha chain.
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| <br/>
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| **<b>Bombyx mori</b> (2 PDB entries): <br>
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| ***[[1gd6]] <div class="pdb-prints 1gd6"></div> | |
| ***Title: STRUCTURE OF THE BOMBYX MORI LYSOZYME | |
| ***2.5 A resolution | | ***2.5 A resolution |
| | == Alternative names for NADP-dependent malic enzyme == |
| | Molecule '''NADP-dependent malic enzyme''', also known as '''NADP-ME''', '''NADP-dependent malic enzyme''' and '''Malic enzyme 1'''. |
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| ***[http://www.ebi.ac.uk/pdbe/entry/search/index?organism_scientific:%34Bombyx%20mori%34&molecule_name:%34Lysozyme%34 Search for other PDB entries] <br/>
| | == 3D Structures of NADP-dependent malic enzyme == |
| *<b>Viruses</b> (578 PDB entries): <br>
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| **<b>Enterobacteria phage P22</b> (2 PDB entries): <br>
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| ***[[2anv]] <div class="pdb-prints 2anv"></div>
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| ***Title: crystal structure of P22 lysozyme mutant L86M
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| ***1.04 A resolution
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| ***[http://www.ebi.ac.uk/pdbe/entry/search/index?organism_scientific:%34Enterobacteria%20phage%20P22%34&molecule_name:%34Lysozyme%34 Search for other PDB entries] <br/>
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| **<b>Enterobacteria phage P21</b> (2 PDB entries): <br>
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| ***[[3hdf]] <div class="pdb-prints 3hdf"></div>
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| ***Title: Crystal structure of truncated endolysin R21 from phage 21
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| ***1.7 A resolution
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| ***[http://www.ebi.ac.uk/pdbe/entry/search/index?organism_scientific:%34Enterobacteria%20phage%20P21%34&molecule_name:%34Lysozyme%34 Search for other PDB entries] <br/>
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| **<b>Enterobacteria phage T4</b> (561 PDB entries): <br>
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| ***[[1swy]] <div class="pdb-prints 1swy"></div>
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| ***Title: Use of a Halide Binding Site to Bypass the 1000-atom Limit to Ab initio Structure Determination
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| ***1.06 A resolution
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| ***[http://www.ebi.ac.uk/pdbe/entry/search/index?organism_scientific:%34Enterobacteria%20phage%20T4%34&molecule_name:%34Lysozyme%34 Search for other PDB entries] <br/>
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| **<b>Escherichia phage T5</b> (1 PDB entries): <br>
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| ***[[2mxz]] <div class="pdb-prints 2mxz"></div>
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| ***Title: Bacteriophage T5 l-alanoyl-d-glutamate peptidase comlpex with Zn2+ (Endo T5-ZN2+)
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| ***N/A A resolution
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| <br/>
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| **<b>Enterobacteria phage lambda</b> (3 PDB entries): <br>
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| ***[[1am7]] <div class="pdb-prints 1am7"></div>
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| ***Title: Lysozyme from bacteriophage lambda
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| ***2.3 A resolution
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| ***[http://www.ebi.ac.uk/pdbe/entry/search/index?organism_scientific:%34Enterobacteria%20phage%20lambda%34&molecule_name:%34Lysozyme%34 Search for other PDB entries] <br/>
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| **<b>Streptococcus phage CP-7</b> (1 PDB entries): <br>
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| ***[[4cvd]] <div class="pdb-prints 4cvd"></div>
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| ***Title: Crystal structure of the central repeat of cell wall binding module of Cpl7
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| ***1.666 A resolution
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| <br/>
| | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} |
| **<b>Enterobacteria phage P1</b> (2 PDB entries): <br>
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| ***[[1xju]] <div class="pdb-prints 1xju"></div>
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| ***Title: Crystal structure of secreted inactive form of P1 phage endolysin Lyz
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| ***1.07 A resolution
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| ***[http://www.ebi.ac.uk/pdbe/entry/search/index?organism_scientific:%34Enterobacteria%20phage%20P1%34&molecule_name:%34Lysozyme%34 Search for other PDB entries] <br/>
| | [[3wja]], [[2aw5]] – hME - human<br /> |
| **<b>Streptococcus phage Cp-1</b> (6 PDB entries): <br>
| | [[7x11]], [[7x12]] – hME1 + NADP<br /> |
| ***[[2j8g]] <div class="pdb-prints 2j8g"></div>
| | [[8e76]], [[8eyn]] – hME3<br /> |
| ***Title: Crystal structure of the modular Cpl-1 endolysin complexed with a peptidoglycan analogue (E94Q mutant in complex with a tetrasaccharide- pentapeptide)
| | [[8e78]], [[8e8o]], [[8eyo]] – hME3 + NADP<br /> |
| ***1.69 A resolution
| | [[1gq2]] – ME + NADP - pigeon<br /> |
| | [[5ou5]] – ME - maize<br /> |
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| ***[http://www.ebi.ac.uk/pdbe/entry/search/index?organism_scientific:%34Streptococcus%20phage%20Cp-1%34&molecule_name:%34Lysozyme%34 Search for other PDB entries] <br/>
| | == References == |
| *<b>Bacteria</b> (1 PDB entries): <br>
| | <references/> |
| **<b>Enterobacteria phage T4</b> (1 PDB entries): <br>
| | [[Category:Topic Page]][[Category:PDBe]] |
| ***[[2qb0]] <div class="pdb-prints 2qb0"></div>
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| ***Title: Structure of the 2TEL crystallization module fused to T4 lysozyme with an Ala-Gly-Pro linker.
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| ***2.56 A resolution
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| ***Other macromolecules also in this entry: Transcription factor ETV6, .
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| <br/>
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| **<b>Escherichia coli</b> (1 PDB entries): <br>
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| ***[[2qb0]] <div class="pdb-prints 2qb0"></div>
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| ***Title: Structure of the 2TEL crystallization module fused to T4 lysozyme with an Ala-Gly-Pro linker.
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| ***2.56 A resolution
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| ***Other macromolecules also in this entry: Transcription factor ETV6, .
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| <br/>
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<StructureSection load='3wja' size='340' side='right' caption='The best structure for NADP-dependent malic enzyme shown: 3wja' scene=>
Best example is PDB entry 3wja and is shown in the viewer.
Catalytic ActivityCatalytic Activity
Oxaloacetate = pyruvate + CO(2).Data source: Uniprot P48163
Or the decarboxylation of malate to pyruvate[1].
Biological processBiological process
Is involved in the following biological processes:
malate metabolic process
oxidation-reduction process
response to carbohydrate
response to hormone
carbohydrate metabolic process
protein tetramerization
regulation of NADP metabolic process
NADP biosynthetic process
small molecule metabolic process
cellular lipid metabolic process
In structuresIn structures
NADP-dependent malic enzyme is found in 3 PDB entries
- Eukaryota (3 PDB entries):
- Homo sapiens (2 PDB entries):
- Columba livia (1 PDB entries):
- 1gq2
- Title: MALIC ENZYME FROM PIGEON LIVER
- 2.5 A resolution
Alternative names for NADP-dependent malic enzymeAlternative names for NADP-dependent malic enzyme
Molecule NADP-dependent malic enzyme, also known as NADP-ME, NADP-dependent malic enzyme and Malic enzyme 1.
3D Structures of NADP-dependent malic enzyme3D Structures of NADP-dependent malic enzyme
Updated on 11-July-2023
3wja, 2aw5 – hME - human
7x11, 7x12 – hME1 + NADP
8e76, 8eyn – hME3
8e78, 8e8o, 8eyo – hME3 + NADP
1gq2 – ME + NADP - pigeon
5ou5 – ME - maize
ReferencesReferences
- ↑ Loeber G, Dworkin MB, Infante A, Ahorn H. Characterization of cytosolic malic enzyme in human tumor cells. FEBS Lett. 1994 May 16;344(2-3):181-6. PMID:8187880