MRNA capping enzyme: Difference between revisions
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<StructureSection load=' | <StructureSection load='4ckb' size='400' side='right' caption='Vaccinia virus mRNA capping enzyme catalytic subunit (pink) and regulatory subunit (yellow) complex with SAH and GTP (PDB code [[4ckb]])' scene='84/844924/Cv/3'> | ||
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== Structural highlights == | == Structural highlights == | ||
<scene name='84/844924/Cv/14'>Vaccinia virus mRNA capping enzyme catalytic subunit and regulatory subunit complex with SAH and GTP</scene> (PDB code [[4ckb]]). The 3D structure of Vaccinia virus Mce shows the regulatory subunit or RNA triphosphatase domain which forms a hydrophilic tunnel. The <scene name='84/844924/Cv/15'>tunnel is lined with charged residues with one half of the internal surface is lined almost exclusively with basic residues (colored in royalblue) and the other half with acidic residues (colored in red)</scene>. The structure shows the catalytic subunit or guanylyltransferase which contains the active site that binds the GTP via <scene name='84/844924/Cv/16'>two motifs</scene> rich in basic residues<ref>PMID:24607143</ref>. | |||
*<scene name='84/844924/Cv/17'>Whole GTP binding site</scene>. Water molecule is shown as red sphere. | |||
*<scene name='84/844924/Cv/18'>Basic residues</scene> (colored in royalblue), which are situated in GTP-binding site. | |||
*<scene name='84/844924/Cv/12'>SAH binding site</scene>. | |||
*<scene name='84/844924/Cv/13'>Stacking interaction between Tyr residue and SAH</scene>. | |||
</StructureSection> | </StructureSection> | ||
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[[5x6y]] - RdvMce + SAM<br /> | [[5x6y]] - RdvMce + SAM<br /> | ||
[[5x70]] - RdvMce + RNA<br /> | [[5x70]] - RdvMce + RNA<br /> | ||
[[8axv]] - cvMce – chikungunya virus – Cryo EM<br /> | |||
[[8aov]] - cvMce + GTP – Cryo EM<br /> | |||
[[7fgg]] - cvMce + 7GMP – Cryo EM<br /> | |||
[[7fgh]], [[7fgi]], [[8aow]] - cvMce + SAH + 7GMP – Cryo EM<br /> | |||
[[7y38]] - cvMce + RNA polymerase + ATP + GTP – Cryo EM<br /> | |||
[[7x01]] - cvMce + inhibitor – Cryo EM<br /> | |||
== References == | == References == | ||
<references/> | <references/> | ||