5e6f: Difference between revisions
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The | ==Canarypox virus resolvase== | ||
<StructureSection load='5e6f' size='340' side='right'caption='[[5e6f]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5e6f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canarypox_virus Canarypox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E6F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E6F FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5e6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e6f OCA], [https://pdbe.org/5e6f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5e6f RCSB], [https://www.ebi.ac.uk/pdbsum/5e6f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5e6f ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q6VZ86_CNPV Q6VZ86_CNPV] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Poxviruses replicate their linear genomes by forming concatemers that must be resolved into monomeric units in order to produce new virions. A viral resolvase cleaves DNA 4-way junctions extruded at the concatemer junctions to produce monomeric genomes. This cleavage reaction is required for viral replication, so the resolvase is an attractive target for small molecule inhibitors. To provide a platform for understanding resolvase mechanism and designing inhibitors, we have determined the crystal structure of the canarypox virus (CPV) resolvase. CPV resolvase is dimer of RNase H superfamily domains related to E. coli RuvC, with an active site lined by highly conserved, acidic residues that bind metal ions. There are several intriguing structural differences between resolvase and RuvC and a model of the CPV resolvase.HJ complex provides insights into the consequences of these differences, including a plausible explanation for the weak sequence specificity exhibited by the poxvirus enzymes. The model also explains why the poxvirus resolvases are more promiscuous than RuvC, cleaving a variety of branched, bulged, and flap containing substrates. Based on the unique active site structure observed for CPV resolvase, we have carried out a series of experiments to test divalent ion usage and preferences. We find that the two resolvase metal binding sites have different preferences for Mg2+vs. Mn2+ Optimal resolvase activity is maintained with 5 muM Mn2+and 100 muM Mg2+, concentrations that are well below those required for either metal alone. Together, our findings provide biochemical insights and structural models that will facilitate studying poxvirus replication and the search for efficient poxvirus inhibitors. | |||
Structure and metal-binding properties of a poxvirus resolvase.,Li H, Hwang Y, Perry K, Bushman F, Van Duyne GD J Biol Chem. 2016 Mar 24. pii: jbc.M115.709139. PMID:27013661<ref>PMID:27013661</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5e6f" style="background-color:#fffaf0;"></div> | ||
[[Category: Hwang | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Resolvase 3D structures|Resolvase 3D structures]] | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Canarypox virus]] | |||
[[Category: Large Structures]] | |||
[[Category: Bushman FD]] | |||
[[Category: Hwang Y]] | |||
[[Category: Li H]] | |||
[[Category: Perry K]] | |||
[[Category: Van Duyne GD]] | |||
Latest revision as of 09:14, 5 July 2023
Canarypox virus resolvaseCanarypox virus resolvase
Structural highlights
FunctionPublication Abstract from PubMedPoxviruses replicate their linear genomes by forming concatemers that must be resolved into monomeric units in order to produce new virions. A viral resolvase cleaves DNA 4-way junctions extruded at the concatemer junctions to produce monomeric genomes. This cleavage reaction is required for viral replication, so the resolvase is an attractive target for small molecule inhibitors. To provide a platform for understanding resolvase mechanism and designing inhibitors, we have determined the crystal structure of the canarypox virus (CPV) resolvase. CPV resolvase is dimer of RNase H superfamily domains related to E. coli RuvC, with an active site lined by highly conserved, acidic residues that bind metal ions. There are several intriguing structural differences between resolvase and RuvC and a model of the CPV resolvase.HJ complex provides insights into the consequences of these differences, including a plausible explanation for the weak sequence specificity exhibited by the poxvirus enzymes. The model also explains why the poxvirus resolvases are more promiscuous than RuvC, cleaving a variety of branched, bulged, and flap containing substrates. Based on the unique active site structure observed for CPV resolvase, we have carried out a series of experiments to test divalent ion usage and preferences. We find that the two resolvase metal binding sites have different preferences for Mg2+vs. Mn2+ Optimal resolvase activity is maintained with 5 muM Mn2+and 100 muM Mg2+, concentrations that are well below those required for either metal alone. Together, our findings provide biochemical insights and structural models that will facilitate studying poxvirus replication and the search for efficient poxvirus inhibitors. Structure and metal-binding properties of a poxvirus resolvase.,Li H, Hwang Y, Perry K, Bushman F, Van Duyne GD J Biol Chem. 2016 Mar 24. pii: jbc.M115.709139. PMID:27013661[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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