5dtu: Difference between revisions
Jump to navigation
Jump to search
m Protected "5dtu" [edit=sysop:move=sysop] |
No edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Crystal structure of the RNA-helicase Prp28 from Chaetomium thermophilum bound to ADP== | |||
<StructureSection load='5dtu' size='340' side='right'caption='[[5dtu]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5dtu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DTU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DTU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.199Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dtu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dtu OCA], [https://pdbe.org/5dtu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dtu RCSB], [https://www.ebi.ac.uk/pdbsum/5dtu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dtu ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G0SBQ7_CHATD G0SBQ7_CHATD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Prp28 (pre-mRNA-splicing ATP-dependent RNA helicase 28) is a spliceosomal DEAD-box helicase which is involved in two steps of spliceosome assembly. It is required for the formation of commitment complex 2 in an ATP-independent manner as well as for the formation of the pre-catalytic spliceosome, which in contrast is ATP-dependent. During the latter step, Prp28 is crucial for the integration of the U4/U6.U5 tri-snRNP since it displaces the U1 snRNP and allows the U6 snRNP to base-pair with the 5'-splice site. Here, the crystal structure of Prp28 from the thermophilic fungus Chaetomium thermophilum is reported at 3.2 A resolution and is compared with the available structures of homologues. | |||
Structural analysis of the spliceosomal RNA helicase Prp28 from the thermophilic eukaryote Chaetomium thermophilum.,Tauchert MJ, Ficner R Acta Crystallogr F Struct Biol Commun. 2016 May 1;72(Pt 5):409-16. doi:, 10.1107/S2053230X16006038. Epub 2016 Apr 22. PMID:27139834<ref>PMID:27139834</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5dtu" style="background-color:#fffaf0;"></div> | ||
[[Category: Ficner | |||
==See Also== | |||
*[[Helicase 3D structures|Helicase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] | |||
[[Category: Large Structures]] | |||
[[Category: Ficner R]] | |||
[[Category: Tauchert MJ]] | |||
Latest revision as of 00:59, 29 June 2023
Crystal structure of the RNA-helicase Prp28 from Chaetomium thermophilum bound to ADPCrystal structure of the RNA-helicase Prp28 from Chaetomium thermophilum bound to ADP
Structural highlights
FunctionPublication Abstract from PubMedPrp28 (pre-mRNA-splicing ATP-dependent RNA helicase 28) is a spliceosomal DEAD-box helicase which is involved in two steps of spliceosome assembly. It is required for the formation of commitment complex 2 in an ATP-independent manner as well as for the formation of the pre-catalytic spliceosome, which in contrast is ATP-dependent. During the latter step, Prp28 is crucial for the integration of the U4/U6.U5 tri-snRNP since it displaces the U1 snRNP and allows the U6 snRNP to base-pair with the 5'-splice site. Here, the crystal structure of Prp28 from the thermophilic fungus Chaetomium thermophilum is reported at 3.2 A resolution and is compared with the available structures of homologues. Structural analysis of the spliceosomal RNA helicase Prp28 from the thermophilic eukaryote Chaetomium thermophilum.,Tauchert MJ, Ficner R Acta Crystallogr F Struct Biol Commun. 2016 May 1;72(Pt 5):409-16. doi:, 10.1107/S2053230X16006038. Epub 2016 Apr 22. PMID:27139834[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||