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== | ==Crystal structure of the Borna Disease Virus Nucleoprotein== | ||
<StructureSection load='1pp1' size='340' side='right'caption='[[1pp1]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1pp1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borna_disease_virus Borna disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PP1 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pp1 OCA], [https://pdbe.org/1pp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pp1 RCSB], [https://www.ebi.ac.uk/pdbsum/1pp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pp1 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/NCAP_BDV1 NCAP_BDV1] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Targets viral NC to the nucleus. Could be involved in the transport of nucleoprotein particles from the nucleus to the cytoplasm. The nuclear export signal is masked by the interaction with the P protein.<ref>PMID:11238866</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Borna disease virus (BDV) causes an infection of the central nervous system in a wide range of vertebrates, which can fatally progress to an immune-mediated disease, called Borna disease. BDV is a member of the Mononegavirales, which also includes the highly infectious measles and Ebola viruses. The viral nucleoproteins are central to transcription, replication, and packaging of the RNA genome. We present the X-ray structure of the BDV nucleoprotein determined at 1.76 A resolution. The structure reveals a novel fold, organized into two distinct domains, and an assembly into a planar homotetramer. Surface potential calculations strongly support an RNA binding model with the RNA wrapping around the outside of the tetramer, although a positively charged central channel in the tetramer could fit single-stranded RNA in an alternative binding mode. This first structure of an RNA virus nucleoprotein provides a paradigmatic model for RNA packaging and replication of single-stranded RNA viruses. | Borna disease virus (BDV) causes an infection of the central nervous system in a wide range of vertebrates, which can fatally progress to an immune-mediated disease, called Borna disease. BDV is a member of the Mononegavirales, which also includes the highly infectious measles and Ebola viruses. The viral nucleoproteins are central to transcription, replication, and packaging of the RNA genome. We present the X-ray structure of the BDV nucleoprotein determined at 1.76 A resolution. The structure reveals a novel fold, organized into two distinct domains, and an assembly into a planar homotetramer. Surface potential calculations strongly support an RNA binding model with the RNA wrapping around the outside of the tetramer, although a positively charged central channel in the tetramer could fit single-stranded RNA in an alternative binding mode. This first structure of an RNA virus nucleoprotein provides a paradigmatic model for RNA packaging and replication of single-stranded RNA viruses. | ||
Crystal structure of the borna disease virus nucleoprotein.,Rudolph MG, Kraus I, Dickmanns A, Eickmann M, Garten W, Ficner R Structure. 2003 Oct;11(10):1219-26. PMID:14527390<ref>PMID:14527390</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1pp1" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Borna disease virus]] | [[Category: Borna disease virus]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Dickmanns | [[Category: Dickmanns A]] | ||
[[Category: Ficner | [[Category: Ficner R]] | ||
[[Category: Garten | [[Category: Garten W]] | ||
[[Category: Kraus | [[Category: Kraus I]] | ||
[[Category: Rudolph | [[Category: Rudolph MG]] | ||
Latest revision as of 14:48, 14 June 2023
Crystal structure of the Borna Disease Virus NucleoproteinCrystal structure of the Borna Disease Virus Nucleoprotein
Structural highlights
FunctionNCAP_BDV1 Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Targets viral NC to the nucleus. Could be involved in the transport of nucleoprotein particles from the nucleus to the cytoplasm. The nuclear export signal is masked by the interaction with the P protein.[1] Publication Abstract from PubMedBorna disease virus (BDV) causes an infection of the central nervous system in a wide range of vertebrates, which can fatally progress to an immune-mediated disease, called Borna disease. BDV is a member of the Mononegavirales, which also includes the highly infectious measles and Ebola viruses. The viral nucleoproteins are central to transcription, replication, and packaging of the RNA genome. We present the X-ray structure of the BDV nucleoprotein determined at 1.76 A resolution. The structure reveals a novel fold, organized into two distinct domains, and an assembly into a planar homotetramer. Surface potential calculations strongly support an RNA binding model with the RNA wrapping around the outside of the tetramer, although a positively charged central channel in the tetramer could fit single-stranded RNA in an alternative binding mode. This first structure of an RNA virus nucleoprotein provides a paradigmatic model for RNA packaging and replication of single-stranded RNA viruses. Crystal structure of the borna disease virus nucleoprotein.,Rudolph MG, Kraus I, Dickmanns A, Eickmann M, Garten W, Ficner R Structure. 2003 Oct;11(10):1219-26. PMID:14527390[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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