7sd4: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==SARS-CoV-2 Nucleocapsid N-terminal domain (N-NTD) protein== | ==SARS-CoV-2 Nucleocapsid N-terminal domain (N-NTD) protein== | ||
<StructureSection load='7sd4' size='340' side='right'caption='[[7sd4 | <StructureSection load='7sd4' size='340' side='right'caption='[[7sd4]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7sd4]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SD4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SD4 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7sd4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SD4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SD4 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sd4 OCA], [https://pdbe.org/7sd4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sd4 RCSB], [https://www.ebi.ac.uk/pdbsum/7sd4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sd4 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sd4 OCA], [https://pdbe.org/7sd4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sd4 RCSB], [https://www.ebi.ac.uk/pdbsum/7sd4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sd4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/NCAP_SARS2 NCAP_SARS2] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 22: | Line 22: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Calero | [[Category: Severe acute respiratory syndrome coronavirus 2]] | ||
[[Category: Calero | [[Category: Calero D]] | ||
[[Category: Gronenborn | [[Category: Calero G]] | ||
[[Category: Lu | [[Category: Gronenborn AM]] | ||
[[Category: Polenova | [[Category: Lu M]] | ||
[[Category: Quinn | [[Category: Polenova T]] | ||
[[Category: Runge | [[Category: Quinn CM]] | ||
[[Category: Russell | [[Category: Runge B]] | ||
[[Category: Sarkar | [[Category: Russell RW]] | ||
[[Category: Zeinalilathori | [[Category: Sarkar S]] | ||
[[Category: Zeinalilathori S]] | |||
Latest revision as of 14:20, 14 June 2023
SARS-CoV-2 Nucleocapsid N-terminal domain (N-NTD) proteinSARS-CoV-2 Nucleocapsid N-terminal domain (N-NTD) protein
Structural highlights
FunctionNCAP_SARS2 Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Publication Abstract from PubMedThe nucleocapsid (N) protein is one of the four structural proteins of the SARS-CoV-2 virus and plays a crucial role in viral genome organization and, hence, replication and pathogenicity. The N-terminal domain (N(NTD)) binds to the genomic RNA and thus comprises a potential target for inhibitor and vaccine development. We determined the atomic-resolution structure of crystalline N(NTD) by integrating solid-state magic angle spinning (MAS) NMR and X-ray diffraction. Our combined approach provides atomic details of protein packing interfaces as well as information about flexible regions as the N- and C-termini and the functionally important RNA binding, beta-hairpin loop. In addition, ultrafast (100 kHz) MAS (1)H-detected experiments permitted the assignment of side-chain proton chemical shifts not available by other means. The present structure offers guidance for designing therapeutic interventions against the SARS-CoV-2 infection. Atomic-Resolution Structure of SARS-CoV-2 Nucleocapsid Protein N-Terminal Domain.,Sarkar S, Runge B, Russell RW, Movellan KT, Calero D, Zeinalilathori S, Quinn CM, Lu M, Calero G, Gronenborn AM, Polenova T J Am Chem Soc. 2022 May 31. doi: 10.1021/jacs.2c03320. PMID:35638584[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||