6cmy: Difference between revisions
New page: '''Unreleased structure''' The entry 6cmy is ON HOLD Authors: Rout, A.K., Wu, X., Strub, M.P., Starich, M.R., Hammer III, J.A., Tjandra, N. Description: Solution NMR Structure Determin... |
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==Solution NMR Structure Determination of Mouse Melanoregulin== | |||
<StructureSection load='6cmy' size='340' side='right'caption='[[6cmy]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6cmy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5kbo 5kbo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CMY FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cmy OCA], [https://pdbe.org/6cmy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cmy RCSB], [https://www.ebi.ac.uk/pdbsum/6cmy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cmy ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/MREG_MOUSE MREG_MOUSE] Plays a role in the incorporation of pigments into hair. May function in membrane fusion and regulate the biogenesis of disk membranes of photoreceptor rod cells.<ref>PMID:15550542</ref> <ref>PMID:17260955</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Melanoregulin (Mreg) is a small, highly charged, multiply palmitoylated protein present on the membrane of melanosomes. Mreg is implicated in the transfer of melanosomes from melanocytes to keratinocytes, and in promoting the microtubule minus end-directed transport of these organelles. The possible molecular function of Mreg was identified by solving its structure using nuclear magnetic resonance (NMR) spectroscopy. Mreg contains six alpha helices forming a fishhook-like fold in which positive and negative charges occupy opposite sides of the protein's surface and sandwich a putative, cholesterol recognition sequence (CRAC motif). Mreg containing a point mutation within its CRAC motif still targets to late endosomes/lysosomes, but no longer promotes their microtubule minus end-directed transport. Moreover, wild-type Mreg does not promote the microtubule minus end-directed transport of late endosomes/lysosomes in cells transiently depleted of cholesterol. Finally, reversing the charge of three clustered acidic residues partially inhibits Mreg's ability to drive these organelles to microtubule minus ends. | |||
The Structure of Melanoregulin Reveals a Role for Cholesterol Recognition in the Protein's Ability to Promote Dynein Function.,Rout AK, Wu X, Starich MR, Strub MP, Hammer JA, Tjandra N Structure. 2018 Aug 8. pii: S0969-2126(18)30255-7. doi:, 10.1016/j.str.2018.07.009. PMID:30174147<ref>PMID:30174147</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6cmy" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Mus musculus]] | |||
[[Category: Hammer III JA]] | |||
[[Category: Rout AK]] | |||
[[Category: Starich MR]] | |||
[[Category: Strub MP]] | |||
[[Category: Tjandra N]] | |||
[[Category: Wu X]] | |||
Latest revision as of 13:40, 14 June 2023
Solution NMR Structure Determination of Mouse MelanoregulinSolution NMR Structure Determination of Mouse Melanoregulin
Structural highlights
FunctionMREG_MOUSE Plays a role in the incorporation of pigments into hair. May function in membrane fusion and regulate the biogenesis of disk membranes of photoreceptor rod cells.[1] [2] Publication Abstract from PubMedMelanoregulin (Mreg) is a small, highly charged, multiply palmitoylated protein present on the membrane of melanosomes. Mreg is implicated in the transfer of melanosomes from melanocytes to keratinocytes, and in promoting the microtubule minus end-directed transport of these organelles. The possible molecular function of Mreg was identified by solving its structure using nuclear magnetic resonance (NMR) spectroscopy. Mreg contains six alpha helices forming a fishhook-like fold in which positive and negative charges occupy opposite sides of the protein's surface and sandwich a putative, cholesterol recognition sequence (CRAC motif). Mreg containing a point mutation within its CRAC motif still targets to late endosomes/lysosomes, but no longer promotes their microtubule minus end-directed transport. Moreover, wild-type Mreg does not promote the microtubule minus end-directed transport of late endosomes/lysosomes in cells transiently depleted of cholesterol. Finally, reversing the charge of three clustered acidic residues partially inhibits Mreg's ability to drive these organelles to microtubule minus ends. The Structure of Melanoregulin Reveals a Role for Cholesterol Recognition in the Protein's Ability to Promote Dynein Function.,Rout AK, Wu X, Starich MR, Strub MP, Hammer JA, Tjandra N Structure. 2018 Aug 8. pii: S0969-2126(18)30255-7. doi:, 10.1016/j.str.2018.07.009. PMID:30174147[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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