Isopenicillin N synthase: Difference between revisions
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<StructureSection load='1blz' size='350' side='right' scene='52/525170/Cv/1' caption='Isopenicilln N synthase complex with ACV and NO, [[1blz]]'> | |||
== Function == | == Function == | ||
'''Isopenicillin N synthase''' (IPNS) is an iron-dependent enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV). IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics. The active site of IPNS contains an Fe atom. The reaction involves the reduction of O2 molecule to H2O<ref>PMID:10537113</ref>. | '''Isopenicillin N synthase''' (IPNS) is an iron-dependent enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV). IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics. The active site of IPNS contains an Fe atom. The reaction involves the reduction of O2 molecule to H2O<ref>PMID:10537113</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
IPNS active site contains Fe+2 and the substrate ACV. | IPNS <scene name='52/525170/Cv/7'>active site contains Fe+2 and the substrate ACV</scene>. The <scene name='52/525170/Cv/8'>Fe+2 ion is hexacoordinated to 3 IPNS side chains, one water molecule, the ACV thiolate moiety and NO</scene><ref>PMID:9194566</ref>. Water molecules shown as red spheres. | ||
</StructureSection> | |||
==3D structures of isopenicillin N synthase== | ==3D structures of isopenicillin N synthase== | ||
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
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**[[1bk0]] - AnIPNS + ACV – ''Aspergillus nidulans''<br /> | **[[1bk0]] - AnIPNS + ACV – ''Aspergillus nidulans''<br /> | ||
**[[2bjs]] - AnIPNS (mutant) + ACV<br /> | **[[2bjs]], [[7zoe]] - AnIPNS (mutant) + ACV<br /> | ||
**[[1qiq]], [[1qjf]], [[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1odm]], [[1odn]], [[1oc1]], [[1uzw]], [[1w03]], [[1w05]], [[2bu9]], [[1w3v]], [[1w3x]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y6f]], [[2y60 | **[[1qiq]], [[1qjf]], [[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1odm]], [[1odn]], [[1oc1]], [[1uzw]], [[1w03]], [[1w05]], [[2bu9]], [[1w3v]], [[1w3x]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y6f]], [[2y60]], [[4bb3]] - AnIPNS + ACV derivative<br /> | ||
**[[2jb4]] - AnIPNS + cyclobutanone analog<br /> | **[[2jb4]] - AnIPNS + cyclobutanone analog<br /> | ||
**[[6y0p]], [[6zao]], [[6zaq]] - AnIPNS + IPN<br /> | |||
**[[1qje]] - AnIPNS + IPN + ACV<br /> | **[[1qje]] - AnIPNS + IPN + ACV<br /> | ||
**[[1blz]] - AnIPNS + NO + ACV<br /> | **[[6y0o]], [[6zae]], [[6zaf]], [[6zag]], [[6zah]], [[6zam]] - AnIPNS + ACV<br /> | ||
**[[6zai]], [[6zaj]], [[6zal]], [[6zap]] - AnIPNS + O2 + ACV<br /> | |||
**[[7poy]] - AnIPNS (mutant) + NO + ACV<br /> | |||
**[[7psw]] - AnIPNS (mutant) + Fe + ACV<br /> | |||
**[[1blz]], [[6zan]] - AnIPNS + NO + ACV<br /> | |||
**[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br /> | **[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br /> | ||
**[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br /> | **[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br /> | ||
* | *Other metal containing IPNS | ||
**[[1ips]] – AnIPNS | **[[1ips]] – AnIPNS + Mn<br /> | ||
**[[6zw8]] - AnIPNS + ACV + Cd<br /> | |||
}} | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category: Topic Page]] | [[Category: Topic Page]] | ||