4y9t: Difference between revisions
Jump to navigation
Jump to search
New page: '''Unreleased structure''' The entry 4y9t is ON HOLD Authors: Vetting, M.W., Al Obaidi, N.F., Toro, R., Morisco, L.L., Benach, J., Koss, J., Wasserman, S.R., Attonito, J.D., Scott Glenn... |
No edit summary |
||
| (6 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==CRYSTAL STRUCTURE OF AN ABC TRANSPORTER SOLUTE BINDING PROTEIN (IPR025997) FROM AGROBACTERIUM VITIS S4 (Avi_5305, TARGET EFI-511224) WITH BOUND ALPHA-D-GLUCOSAMINE== | ||
<StructureSection load='4y9t' size='340' side='right'caption='[[4y9t]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4y9t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_vitis_S4 Agrobacterium vitis S4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y9T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y9T FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PA1:2-AMINO-2-DEOXY-ALPHA-D-GLUCOPYRANOSE'>PA1</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y9t OCA], [https://pdbe.org/4y9t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y9t RCSB], [https://www.ebi.ac.uk/pdbsum/4y9t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y9t ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B9K0Q5_AGRVS B9K0Q5_AGRVS] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The uptake of exogenous solutes by prokaryotes is mediated by transport systems embedded in the plasma membrane. In many cases, a solute-binding protein (SBP) is utilized to bind ligands with high affinity and deliver them to the membrane-bound components responsible for translocation into the cytoplasm. In the present study, Avi_5305, an Agrobacterium vitis SBP belonging to Pfam13407, was screened by differential scanning fluorimetry (DSF) and found to be stabilized by D-glucosamine and D-galactosamine. Avi_5305 is the first protein from Pfam13407 shown to be specific for amino sugars, and co-crystallization resulted in structures of Avi_5305 bound to D-glucosamine and D-galactosamine. Typical of Pfam13407, Avi_5305 consists of two alpha/beta domains linked through a hinge region, with the ligand-binding site located in a cleft between the two domains. Comparisons with Escherichia coli ribose-binding protein suggest that a cation-pi interaction with Tyr168 provides the specificity for D-glucosamine/D-galactosamine over D-glucose/D-galactose. | |||
Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine.,Yadava U, Vetting MW, Al Obaidi N, Carter MS, Gerlt JA, Almo SC Acta Crystallogr F Struct Biol Commun. 2016 Jun 1;72(Pt 6):467-72. doi:, 10.1107/S2053230X16007500. Epub 2016 May 23. PMID:27303900<ref>PMID:27303900</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 4y9t" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Agrobacterium vitis S4]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Al Obaidi NF]] | ||
[[Category: | [[Category: Almo SC]] | ||
[[Category: | [[Category: Attonito JD]] | ||
[[Category: | [[Category: Benach J]] | ||
[[Category: | [[Category: Chamala S]] | ||
[[Category: | [[Category: Chowdhury S]] | ||
[[Category: Seidel | [[Category: Gerlt JA]] | ||
[[Category: | [[Category: Koss J]] | ||
[[Category: | [[Category: Lafleur J]] | ||
[[Category: | [[Category: Love J]] | ||
[[Category: | [[Category: Morisco LL]] | ||
[[Category: Scott Glenn A]] | |||
[[Category: Seidel RD]] | |||
[[Category: Toro R]] | |||
[[Category: Vetting MW]] | |||
[[Category: Wasserman SR]] | |||
[[Category: Whalen KL]] | |||
Latest revision as of 11:04, 3 May 2023
CRYSTAL STRUCTURE OF AN ABC TRANSPORTER SOLUTE BINDING PROTEIN (IPR025997) FROM AGROBACTERIUM VITIS S4 (Avi_5305, TARGET EFI-511224) WITH BOUND ALPHA-D-GLUCOSAMINECRYSTAL STRUCTURE OF AN ABC TRANSPORTER SOLUTE BINDING PROTEIN (IPR025997) FROM AGROBACTERIUM VITIS S4 (Avi_5305, TARGET EFI-511224) WITH BOUND ALPHA-D-GLUCOSAMINE
Structural highlights
FunctionPublication Abstract from PubMedThe uptake of exogenous solutes by prokaryotes is mediated by transport systems embedded in the plasma membrane. In many cases, a solute-binding protein (SBP) is utilized to bind ligands with high affinity and deliver them to the membrane-bound components responsible for translocation into the cytoplasm. In the present study, Avi_5305, an Agrobacterium vitis SBP belonging to Pfam13407, was screened by differential scanning fluorimetry (DSF) and found to be stabilized by D-glucosamine and D-galactosamine. Avi_5305 is the first protein from Pfam13407 shown to be specific for amino sugars, and co-crystallization resulted in structures of Avi_5305 bound to D-glucosamine and D-galactosamine. Typical of Pfam13407, Avi_5305 consists of two alpha/beta domains linked through a hinge region, with the ligand-binding site located in a cleft between the two domains. Comparisons with Escherichia coli ribose-binding protein suggest that a cation-pi interaction with Tyr168 provides the specificity for D-glucosamine/D-galactosamine over D-glucose/D-galactose. Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine.,Yadava U, Vetting MW, Al Obaidi N, Carter MS, Gerlt JA, Almo SC Acta Crystallogr F Struct Biol Commun. 2016 Jun 1;72(Pt 6):467-72. doi:, 10.1107/S2053230X16007500. Epub 2016 May 23. PMID:27303900[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||