GMP synthase: Difference between revisions
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<StructureSection load='2vxo' size='400' side='right' caption='Structure of human GMP synthase dimer complex with xantosine 5-phosphate | <StructureSection load='2vxo' size='400' side='right' caption='Structure of human GMP synthase dimer complex with xantosine 5-phosphate and sulfate (PDB entry [[2vxo]])' scene='51/516475/Cv/1'> | ||
== Function == | == Function == | ||
'''GMP synthase''' (GMPS) catalyzes the conversion of xantosine 5’-phosphate (XMP), ATP, glutamine and water to glutamate, GMP, AMP and diphosphate. GMPS is active in purine and glutamate metabolism<ref>PMID:24462112</ref>. GMPS is a bifunctional two-domain enzyme with the N-terminal glutaminase dominates extracts ammonia from glutamine and the C-terminal synthetase adds amine group to XMP to produce GMP. | '''GMP synthase''' (GMPS) catalyzes the conversion of xantosine 5’-phosphate (XMP), ATP, glutamine and water to glutamate, GMP, AMP and diphosphate. GMPS is active in purine and glutamate metabolism<ref>PMID:24462112</ref>. GMPS is a bifunctional two-domain enzyme with the <scene name='51/516475/Cv/12'>N-terminal glutaminase</scene> dominates extracts ammonia from glutamine and the <scene name='51/516475/Cv/13'>C-terminal synthetase</scene> adds amine group to XMP to produce GMP. | ||
== Structural highlights == | == Structural highlights == | ||
Human GMPS structure contains 2 dimerization domains (D1 and D2). The active site is located between the synthetase domain and D2 domain and covered by the LID motif. | Human GMPS structure contains <scene name='51/516475/Cv/14'>2 dimerization domains (D1 and D2)</scene>. The <scene name='51/516475/Cv/15'>active site is located between the synthetase domain and D2 domain and covered by the LID motif</scene>. <scene name='51/516475/Cv/16'>Whole active site</scene>. N-terminal glutaminase domain contains <scene name='51/516475/Cv/17'>catalytic triad Cys104, His190, Glu192</scene><ref>PMID:23816837</ref>. | ||
==3D structures of GMP synthase== | ==3D structures of GMP synthase== | ||
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
[[2vpi]] – hGMPS glutaminase domain – human<br /> | |||
[[2vxo]] - hGMPS + XMP<br /> | |||
[[1wl8]], [[2d7j]] – PhGMPS subunit A – ''Pyrococcus horikoshii''<br /> | [[1wl8]], [[2d7j]] – PhGMPS subunit A – ''Pyrococcus horikoshii''<br /> | ||
[[2dpl]], [[3a4i]] - PhGMPS subunit B<br /> | [[2dpl]], [[3a4i]] - PhGMPS subunit B<br /> | ||
[[2ywb]] – TtGMPS – ''Thermus thermophilus''<br /> | [[2ywb]] – TtGMPS – ''Thermus thermophilus''<br /> | ||
[[2ywc]] – TtGMPS + XMP<br /> | |||
[[3tqi]] – GMPS – ''Coxiella burnetii''<br /> | [[3tqi]] – GMPS – ''Coxiella burnetii''<br /> | ||
[[1kxj]] – TmGMPS – ''Thermotoga maritima''<br /> | [[1kxj]] – TmGMPS – ''Thermotoga maritima''<br /> | ||
[[2iss]] – TmGMPS subunit PDXT + PLP biosynthesis lyase PDXS<br /> | |||
[[2a9v]] – GMPS – ''Thermoplasma acidophilum''<br /> | [[2a9v]] – GMPS – ''Thermoplasma acidophilum''<br /> | ||
[[2lxn]] – | [[2lxn]], [[7d40]], [[7d95]] – MjGMPS subunit A – ''Methanocaldococcus jannaschii''<br /> | ||
[[ | [[7d96]], [[7d97]] – MjGMPS subunit A (mutant)<br /> | ||
[[ | [[6jp9]] – MjGMPS subunit B + XMP<br /> | ||
[[ | [[1gpm]] – GMPS + pyrophosphate + AMP – ''Escherichia coli''<br /> | ||
[[ | [[5tw7]] – GMPS – ''Neisseria gonorrhoeae''<br /> | ||
[[ | [[7mo6]] – GMPS – ''Aspergillus fumigatus''<br /> | ||
[[7sbc]] – GMPS – ''Acinetobacter baumannii''<br /> | |||
== References == | == References == | ||
<references/> | <references/> | ||
</StructureSection> | </StructureSection> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||