4uzm: Difference between revisions
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==Shotgun proteolysis: A practical application== | ==Shotgun proteolysis: A practical application== | ||
<StructureSection load='4uzm' size='340' side='right' caption='[[4uzm | <StructureSection load='4uzm' size='340' side='right'caption='[[4uzm]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4uzm]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZM OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4uzm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UZM FirstGlance]. <br> | ||
</td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uzm OCA], [https://pdbe.org/4uzm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uzm RCSB], [https://www.ebi.ac.uk/pdbsum/4uzm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uzm ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/IGAA_ECOLI IGAA_ECOLI] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4uzm" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Allen MD]] | ||
[[Category: | [[Category: Bycroft M]] | ||
[[Category: | [[Category: Christ D]] | ||
[[Category: Freund SMV]] | |||
Latest revision as of 10:54, 29 March 2023
Shotgun proteolysis: A practical applicationShotgun proteolysis: A practical application
Structural highlights
FunctionPublication Abstract from PubMedWe have previously reported a phage display method for the identification of protein domains on a genome-wide scale (shotgun proteolysis). Here we present the solution structure of a fragment of the Escherichia coli membrane protein yrfF, as identified by shotgun proteolysis, and determined by NMR spectroscopy. Despite the absence of computational predictions, the fragment formed a well-defined beta-barrel structure, distantly falling within the OB-fold classification. Our results highlight the potential of high-throughput experimental approaches for the identification of protein domains for structural studies. Solution structure of a soluble fragment derived from a membrane protein by shotgun proteolysis.,Allen MD, Christie M, Jones P, Porebski BT, Roome B, Freund SM, Buckle AM, Bycroft M, Christ D Protein Eng Des Sel. 2015 Apr 15. pii: gzv021. PMID:25877662[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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