4uzm: Difference between revisions

New page: ==Shotgun proteolysis: A practical application== <StructureSection load='4uzm' size='340' side='right' caption='4uzm, 20 NMR models' scene=''> == Structur...
 
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==Shotgun proteolysis: A practical application==
==Shotgun proteolysis: A practical application==
<StructureSection load='4uzm' size='340' side='right' caption='[[4uzm]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='4uzm' size='340' side='right'caption='[[4uzm]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4uzm]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UZM FirstGlance]. <br>
<table><tr><td colspan='2'>[[4uzm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UZM FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uzw|4uzw]], [[4uzx|4uzx]]</td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uzm OCA], [https://pdbe.org/4uzm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uzm RCSB], [https://www.ebi.ac.uk/pdbsum/4uzm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uzm ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uzm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uzm RCSB], [http://www.ebi.ac.uk/pdbsum/4uzm PDBsum]</span></td></tr>
</table>
<table>
== Function ==
[https://www.uniprot.org/uniprot/IGAA_ECOLI IGAA_ECOLI]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We have previously reported a phage display method for the identification of protein domains on a genome-wide scale (shotgun proteolysis). Here we present the solution structure of a fragment of the Escherichia coli membrane protein yrfF, as identified by shotgun proteolysis, and determined by NMR spectroscopy. Despite the absence of computational predictions, the fragment formed a well-defined beta-barrel structure, distantly falling within the OB-fold classification. Our results highlight the potential of high-throughput experimental approaches for the identification of protein domains for structural studies.
 
Solution structure of a soluble fragment derived from a membrane protein by shotgun proteolysis.,Allen MD, Christie M, Jones P, Porebski BT, Roome B, Freund SM, Buckle AM, Bycroft M, Christ D Protein Eng Des Sel. 2015 Apr 15. pii: gzv021. PMID:25877662<ref>PMID:25877662</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4uzm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Allen, M D.]]
[[Category: Escherichia coli]]
[[Category: Bycroft, M.]]
[[Category: Large Structures]]
[[Category: Christ, D.]]
[[Category: Allen MD]]
[[Category: Freund, S M.V.]]
[[Category: Bycroft M]]
[[Category: Structural protein]]
[[Category: Christ D]]
[[Category: Freund SMV]]

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