2n5u: Difference between revisions
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==Solution structure of the cyanobacterial cytochrome b6f complex subunit PetP== | ==Solution structure of the cyanobacterial cytochrome b6f complex subunit PetP== | ||
<StructureSection load='2n5u' size='340' side='right' caption='[[2n5u | <StructureSection load='2n5u' size='340' side='right'caption='[[2n5u]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2n5u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N5U OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[2n5u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N5U FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n5u OCA], [https://pdbe.org/2n5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n5u RCSB], [https://www.ebi.ac.uk/pdbsum/2n5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n5u ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8DLG9_THEVB Q8DLG9_THEVB] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Thermosynechococcus vestitus BP-1]] | ||
[[Category: | [[Category: Ikegami T]] | ||
[[Category: | [[Category: Roegner M]] | ||
[[Category: | [[Category: Stoll R]] | ||
[[Category: | [[Category: Veit S]] | ||
Latest revision as of 13:21, 15 March 2023
Solution structure of the cyanobacterial cytochrome b6f complex subunit PetPSolution structure of the cyanobacterial cytochrome b6f complex subunit PetP
Structural highlights
FunctionPublication Abstract from PubMedPetP is a peripheral subunit of the cytochrome b6f complex (b6f) present in both, cyanobacteria and red algae. It is bound to the cytoplasmic surface of this membrane protein complex where it greatly affects the efficiency of the linear photosynthetic electron flow although it is not directly involved in the electron transfer reactions. Despite the crystal structures of the b6f core complex, structural information for the transient regulatory b6f subunits is still missing. Here we present the first structure of PetP at atomic resolution as determined by solution NMR. The protein adopts an SH3 fold, which is a common protein motif in eukaryotes but comparatively rare in prokaryotes. The structure of PetP enabled the identification of the potential interaction site for b6f binding by conservation mapping. The interaction surface is mainly formed by two large loop regions and one short 310 helix which also exhibit an increased flexibility as indicated by heteronuclear steady-state {1H}-15N NOE and random coil index parameters. The properties of this potential b6f binding site greatly differ from the canonical peptide binding site which is highly conserved in eukaryotic SH3 domains. Interestingly, three other proteins of the photosynthetic electron transport chain share this SH3 fold with PetP: NdhS of the photosynthetic NADH dehydrogenase-like complex (NDH-1), PsaE of the photosystem 1 and subunit alpha of the ferredoxin-thioredoxin reductase have, similar to PetP, a great impact on the photosynthetic electron transport. Finally, a model is presented to illustrate how SH3 domains modulate the photosynthetic electron transport processes in cyanobacteria. The cyanobacterial cytochrome bf subunit PetP adopts an SH3 fold in solution.,Veit S, Nagadoi A, Rogner M, Rexroth S, Stoll R, Ikegami T Biochim Biophys Acta. 2016 Mar 29;1857(6):705-714. doi:, 10.1016/j.bbabio.2016.03.023. PMID:27033306[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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