Flavin reductase: Difference between revisions

Latest revision as of 12:10, 6 March 2023

Function

Flavin reductase (FR) catalyzes the NADPH-dependent reduction of a variety of flavins such as riboflavin, FAD, FMN, biliverdin, methemoglobin and pyrroloquinoline quinone. FR converts reduced riboflavin to riboflavin and bilirubin to biliverdin. FR is involved in heme catabolism^[1] .

Relevance

FR catalytic reaction is the basis of the therapeutic use of methylene blue or riboflavin in the treatment of methemoglobinemia. Bacterial FR are used to sensitize tumors to drugs.

Structural insights

The because the binding is achieved mainly via hydrophobic interactions (Hydrophobic, Polar).^[2]

Human flavin reductase complex with biliverdin IX α and NADP (PDB code 1he2)

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3D structures of flavin reductase3D structures of flavin reductase

Updated on 06-March-2023

ReferencesReferences

↑ Yubisui T, Takeshita M, Yoneyama Y. Reduction of methemoglobin through flavin at the physiological concentration by NADPH-flavin reductase of human erythrocytes. J Biochem. 1980 Jun;87(6):1715-20. PMID:7400118
↑ Pereira PJ, Macedo-Ribeiro S, Parraga A, Perez-Luque R, Cunningham O, Darcy K, Mantle TJ, Coll M. Structure of human biliverdin IXbeta reductase, an early fetal bilirubin IXbeta producing enzyme. Nat Struct Biol. 2001 Mar;8(3):215-20. PMID:11224564 doi:10.1038/84948

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

[1] Yubisui T, Takeshita M, Yoneyama Y. Reduction of methemoglobin through flavin at the physiological concentration by NADPH-flavin reductase of human erythrocytes. J Biochem. 1980 Jun;87(6):1715-20. PMID:7400118

[2] Pereira PJ, Macedo-Ribeiro S, Parraga A, Perez-Luque R, Cunningham O, Darcy K, Mantle TJ, Coll M. Structure of human biliverdin IXbeta reductase, an early fetal bilirubin IXbeta producing enzyme. Nat Struct Biol. 2001 Mar;8(3):215-20. PMID:11224564 doi:10.1038/84948

[1]

[2]

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+<StructureSection load='' size='350' side='right' scene='49/490853/Cv/1' caption='Human flavin reductase complex with biliverdin IX α and NADP (PDB code [[1he2]]) '>
-<StructureSection load='1he2' size='350' side='right' scene='' caption='Human flavin reductase complex with biliverdin IX α and NADP (PDB code [[1he2]]) '>
-<!--
-Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page.
-Or use the four-green-boxes-button to insert scrollable text adjacent
-to a Jmol applet. Check out the other buttons as well!
--->
 == Function ==
@@ Line 15: / Line 8: @@
 FR catalytic reaction is the basis of the therapeutic use of methylene blue or riboflavin in the treatment of methemoglobinemia.  Bacterial FR are used to sensitize tumors to drugs.
+== Structural insights ==
+The <scene name='49/490853/Cv/4'>NADP containing active site can accommodate a variety of ligands</scene> because the binding is achieved mainly via hydrophobic interactions ({{Template:ColorKey_Hydrophobic}},  {{Template:ColorKey_Polar}}).<ref>PMID:11224564</ref>
 </StructureSection>
@@ Line 20: / Line 17: @@
 Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+{{#tree:id=OrganizedByTopic|openlevels=0|
+*NADPH-dependent Flavin reductase or biliverdin IX beta reductase
+**[[1hdo]], [[7er6]] - hFR + NADP - human<br />
+**[[6opl]] – hFR (mutant) + NADP <br />
+**[[1he2]] - hFR + biliverdin IX α + NADP<BR />
+**[[1he3]] - hFR + mesobiliverdin IV α + NADP<BR />
+**[[1he4]] - hFR + FMN + NADP<BR />
+**[[1he5]] - hFR + lumichrome + NADP<BR />
+**[[5ooh]] – hFR + NADP + erythrosine<br />
+**[[5oog]] – hFR + NADP + phloxine + cyanosine<br />
+**[[7er7]], [[7er8]], [[7er9]], [[7era]], [[7erb]], [[7erc]], [[7erd]], [[7ere]] – hFR + NADP + drug<br />
+**[[3pft]] - FR (mutant) + FMN – ''Mycobacterium goodii''<br />
+**[[5cho]] - FR + FAD – uncultured bacteria<br />
+**[[1qfj]] – FR – ''Escherichia coli''<br />
+**[[1bkj]] – VhFR + FMN – ''Vibrio harveyi''<br />
+**[[2bkj]] - VhFR + NAD + FMN<br />
+*NADH-dependent Flavin reductase
-[[1qfj]] – FR – ''Escherichia coli''<br />
+**[[1rz0]] – GtFR + FAD – ''Geobacillus thermoglucosidasius''<br />
-[[1bkj]] – VhFR + FMN – ''Vibrio harveyi''<br />
+**[[1rz1]] - GtFR + FAD + NAD<br />
-[[2bkj]] - VhFR + NAD + FMN<br />
+**[[5n6q]] - PpFR + FMN – ''Pseudomonas putida''<br />
-[[1hdo]] - hFR + NADP - human<br />
+**[[4uth]] - PpFR (mutant) + FMN <br />
-[[1he2]] - hFR + biliverdin IX α + NADP<BR />
+**[[5lnj]], [[5lni]], [[4uti]], [[4utj]] - PpFR (mutant) + FMN derivative + coumarin derivative<br />
-[[1he3]] - hFR + mesobiliverdin IV α + NADP<BR />
+**[[2d36]] - StFR + FMN – ''Sulfurisphaera tokodaii''<br />
-[[1he4]] - hFR + FMN + NADP<BR />
+**[[2d37]] - StFR + FMN + NAD<br />
-[[1he5]] - hFR + lumichrome + NADP<BR />
+**[[2d38]] - StFR + FMN + NADP<br />
-[[1rz0]] – GtFR + FAD – ''Geobacillus thermoglucosidasius''<br />
-[[1rz1]] - GtFR + FAD + NAD<br />
-[[3pft]] - FR (mutant) + FMN – ''Mycobacterium goodii''
+}}
 == References ==
 <references/>