6fuw: Difference between revisions
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New page: '''Unreleased structure''' The entry 6fuw is ON HOLD Authors: Clerici, M., Faini, M., Jinek, M. Description: Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PA... |
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==Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolution== | |||
<SX load='6fuw' size='340' side='right' viewer='molstar' caption='[[6fuw]], [[Resolution|resolution]] 3.07Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6fuw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Unidentified_adenovirus Unidentified adenovirus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6fbs 6fbs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FUW FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fuw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fuw OCA], [https://pdbe.org/6fuw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fuw RCSB], [https://www.ebi.ac.uk/pdbsum/6fuw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fuw ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-A-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation. | |||
Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.,Clerici M, Faini M, Muckenfuss LM, Aebersold R, Jinek M Nat Struct Mol Biol. 2018 Jan 22. pii: 10.1038/s41594-017-0020-6. doi:, 10.1038/s41594-017-0020-6. PMID:29358758<ref>PMID:29358758</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6fuw" style="background-color:#fffaf0;"></div> | ||
[[Category: Faini | |||
[[Category: | ==See Also== | ||
*[[WD-repeat protein 3D structures|WD-repeat protein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Unidentified adenovirus]] | |||
[[Category: Clerici M]] | |||
[[Category: Faini M]] | |||
[[Category: Jinek M]] | |||
Latest revision as of 08:57, 2 March 2023
Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolutionCryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolution
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