Ets1: Difference between revisions

Latest revision as of 14:29, 23 February 2023

Function

Ets1 (E26 Transformation-Specific) or p54 is a proto-oncogene translation factor. Ets1 contributes to the regulation of cellular differentiation^[1] .

Relevance

Ets1 promotes invasive behavior in endothelial cells, smooth muscle cells and epithelial cancer cells.

Structural highlights

Ets1 contains an ETS domain (residues 332-415) which is a helix-turn-helix DNA-binding domain which recognizes the sequence GGAA/T. The ETS domain is flanked by autoinhibitory domains. At the N-terminal, Ets1 contains a pointed domain (PNT) (residues 54-135) and a MAP kinase phosphorylation domain. PNT domain is related to SAM domains and contains 4 α-helices.

(PDB code 1mdm).^[2]

Structure of mouse Ets1 ETS domain (green) complex with paired box protein Pax5 (magenta) and MB-1 promoter DNA (PDB code 1mdm).

Drag the structure with the mouse to rotate

3D Structures of Ets13D Structures of Ets1

Updated on 23-February-2023

ReferencesReferences

↑ Dwyer J, Li H, Xu D, Liu JP. Transcriptional regulation of telomerase activity: roles of the the Ets transcription factor family. Ann N Y Acad Sci. 2007 Oct;1114:36-47. PMID:17986575 doi:http://dx.doi.org/10.1196/annals.1396.022
↑ Garvie CW, Pufall MA, Graves BJ, Wolberger C. Structural analysis of the autoinhibition of Ets-1 and its role in protein partnerships. J Biol Chem. 2002 Nov 22;277(47):45529-36. Epub 2002 Sep 6. PMID:12221090 doi:10.1074/jbc.M206327200

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Michal Harel, Alexander Berchansky, Jaime Prilusky

[1] Dwyer J, Li H, Xu D, Liu JP. Transcriptional regulation of telomerase activity: roles of the the Ets transcription factor family. Ann N Y Acad Sci. 2007 Oct;1114:36-47. PMID:17986575 doi:http://dx.doi.org/10.1196/annals.1396.022

[2] Garvie CW, Pufall MA, Graves BJ, Wolberger C. Structural analysis of the autoinhibition of Ets-1 and its role in protein partnerships. J Biol Chem. 2002 Nov 22;277(47):45529-36. Epub 2002 Sep 6. PMID:12221090 doi:10.1074/jbc.M206327200

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[2]

@@ Line 1: / Line 1: @@
+<StructureSection load='1mdm' size='340' side='right' caption='Structure of mouse Ets1 ETS domain (green) complex with paired box protein Pax5 (magenta) and MB-1 promoter DNA (PDB code [[1mdm]]).' scene='59/595751/Cv/1'>
-<StructureSection load='1mdm' size='340' side='right' caption='Structure of mouse Ets1 ETS domain complex with paired box protein Pax5 and MB-1 promoter DNA (PDB code [[1mdm]]).' scene=''>
 == Function ==
+'''Ets1 (E26 Transformation-Specific)''' or '''p54''' is a proto-oncogene translation factor.  Ets1 contributes to the regulation of cellular differentiation<ref>PMID:17986575</ref>  .
-'''Ets1 (E26 Transformation-Specific)''' is a proto-oncogene translation factor.  Ets1 contributes to the regulation of cellular differentiation.  Ets1 contains an ETS domain which is a helix-turn-helix DNA-binding domain which recognizes the sequence GGAA/T.  The ETS domain is flanked by autoinhibitory domains.  At the N-terminal, Ets1 contains a pointed domain (PNT) and a MAP kinase phosphorylation domain.  PNT domain is related to SAM domains and contains 4 α-helices.
-== Disease ==
 == Relevance ==
 Ets1 promotes invasive behavior in endothelial cells, smooth muscle cells and epithelial cancer cells.
+== Structural highlights ==
+Ets1 contains an ETS domain (residues 332-415) which is a helix-turn-helix DNA-binding domain which recognizes the sequence GGAA/T.  The ETS domain is flanked by autoinhibitory domains.  At the N-terminal, Ets1 contains a pointed domain (PNT) (residues 54-135) and a MAP kinase phosphorylation domain.  PNT domain is related to SAM domains and contains 4 α-helices.
-== Structural highlights ==
+<scene name='59/595751/Cv/3'>Ets1 ETS domain with paired box protein Pax5 and MB-1 promoter DNA</scene> (PDB code [[1mdm]]).<ref>PMID:12221090</ref>
+</StructureSection>
 == 3D Structures of Ets1 ==
 Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+{{#tree:id=OrganizedByTopic|openlevels=0|
-[[1md0]] – mETS1 ETS domain - mouse<br />
+*Ets1
-[[1r36]] – mETS1 ETS and autoinhibitory domains - NMR<br />
-[[2jv3]], [[2kmd]] – mETS1 PNT domain - NMR<br />
-[[1gvj]] – hETS1 ETS and autoinhibitory domains - human<br />
-===Ets1 binary complexes===
+**[[1gvj]] – hETS1 ETS and autoinhibitory domains - human<br />
+**[[1md0]] – mETS1 ETS domain - mouse<br />
+**[[1r36]] – mETS1 ETS and autoinhibitory domains - NMR<br />
+**[[2jv3]], [[2kmd]] – mETS1 PNT domain - NMR<br />
-[[2nny]] – hETS1 + DNA <br />
+*Ets1 binary complexes
-[[3mfk]] – hETS1 + stromelysin promoter DNA <br />
-[[3ri4]] – hETS1 + TCR α promoter DNA <br />
-[[2stt]], [[2stw]] – hETS1 + DNA - NMR<br />
-[[1k79]], [[1k7a]] – mETS1 ETS domain + DNA containing GGAA - mouse<br />
-===Ets1 ternary complexes===
+**[[2nny]] – hETS1 residues 280-441 + DNA <br />
+**[[3mfk]] – hETS1 residues 280-441 + stromelysin promoter DNA <br />
+**[[3ri4]] – hETS1 residues 280-441 + TCR α promoter DNA <br />
+**[[2stt]], [[2stw]] – hETS1 ETS domain + DNA - NMR<br />
+**[[1k79]], [[1k7a]] – mETS1 ETS domain + DNA containing GGAA<br />
+**[[6da1]], [[6dat]] – mETS1 + SSR peptide <br />
-[[1mdm]] – mETS1 ETS domain + paired box protein Pax5 + MB-1 promoter DNA <br />
+*Ets1 higher complexes
-[[1k78]] – hETS1 ETS domain + paired box protein Pax5 + MB-1 promoter DNA - NMR<br />
-[[4l0y]], [[4l0z]], [[4l18]] – hETS1 + TCR α promoter DNA + RunT-related transcription factor<br />
-[[4lg0]] – hETS1 + cadherin promoter DNA + forkhead box protein<br />
+**[[1k78]] – hETS1 ETS domain + paired box protein Pax5 + MB-1 promoter DNA - NMR<br />
+**[[4l0y]], [[4l0z]], [[4l18]] – hETS1 residues 296-441 + TCR α promoter DNA + RunT-related transcription factor<br />
+**[[4lg0]] – hETS1 residues 280-441 + cadherin promoter DNA + forkhead box protein<br />
+**[[1mdm]] – mETS1 ETS domain + paired box protein Pax5 + MB-1 promoter DNA <br />
+**[[3wu1]] – hETS1 276-441 (mutant) + RUNX1 + DNA <br />
+**[[3wts]], [[3wtt]], [[3wtu]], [[3wtv]], [[3wtw]], [[3wtx]], [[3wty]] – hETS1 276-441 (mutant) + RUNX1 + CBF beta + DNA <br />
+}}
 == References ==
 <references/>
 [[Category:Topic Page]]