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EPSP synthase: Difference between revisions

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<StructureSection load='' size='350' side='right' caption='Structure of E. coli EPSP synthase complex with shikimate-3-phosphate, the herbicide glyphosate and formic acid (PDB entry [[1g6s]])' scene='57/570585/Cv/1'>
<StructureSection load='' size='350' side='right' caption='Structure of E. coli EPSP synthase complex with shikimate-3-phosphate, the herbicide glyphosate and formic acid (PDB entry [[1g6s]])' scene='57/570585/Cv/1'>
__TOC__
== Function ==
== Function ==


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The enzyme has two domains, with the active site found in the interdomain cleft <scene name='57/570585/Two_domains/5'>(open conformation)</scene>.  There is a substantial structural change upon substrate binding, resulting in a <scene name='57/570585/Closed_formation/3'>closed</scene> conformation. <scene name='57/570585/Cv/9'>See animation of this process</scene>. '''Glyphosate''' (also known as '''Roundup''') occupies the <scene name='57/570585/Glyphosate_s3p/1'>binding site</scene> of the second substrate, phosphoenol pyruvate <ref>PMID:11171958</ref>.  Interestingly CP4 EPSP synthase still binds glyphosate in the absence of PEP, but a conformational change in glyphosate to accommodate a steric clash with <scene name='57/570585/Glyphosate_s3p_distance/2'>Glu 354</scene> shortens the length of glyphosate, from 7.3 angstroms to 6.67 angstroms, and changes the IC50 by a factor of over 4,000, from 2.5 micromolar to 11 millimolar.   
The enzyme has two domains, with the active site found in the interdomain cleft <scene name='57/570585/Two_domains/5'>(open conformation)</scene>.  There is a substantial structural change upon substrate binding, resulting in a <scene name='57/570585/Closed_formation/3'>closed</scene> conformation. <scene name='57/570585/Cv/9'>See animation of this process</scene>. '''Glyphosate''' (also known as '''Roundup''') occupies the <scene name='57/570585/Glyphosate_s3p/1'>binding site</scene> of the second substrate, phosphoenol pyruvate <ref>PMID:11171958</ref>.  Interestingly CP4 EPSP synthase still binds glyphosate in the absence of PEP, but a conformational change in glyphosate to accommodate a steric clash with <scene name='57/570585/Glyphosate_s3p_distance/2'>Glu 354</scene> shortens the length of glyphosate, from 7.3 angstroms to 6.67 angstroms, and changes the IC50 by a factor of over 4,000, from 2.5 micromolar to 11 millimolar.   
</StructureSection>


===3D structures of EPSP synthase ===
==3D structures of EPSP synthase ==


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
[[EPSP synthase 3D structures]]
{{#tree:id=OrganizedByTopic|openlevels=0|
</StructureSection>


*5-enolpyruvylshikimate 3-phosphate (EPSP) synthase
**[[7pxy]] – EPSP – ''Arabidopsis thaliana''<br />
**[[1eps]] – EcEPSP – ''Escherichia coli''<br />
**[[1p88]], [[1p89]] - EcEPSP N terminal - NMR<br />
**[[1rf5]] - SpEPSP – ''Streptococcus pneumoniae''<br />
**[[7m0o]] - EPSP – ''Streptococcus sviceus''<br />
**[[2bjb]], [[2o15]] – MtEPSP – ''Mycobacterium tuberculosis''<br />
**[[2gg4]] – AgEPSP – ''Agrobacterium''<br />
**[[3roi]], [[3tr1]], [[4gfp]] – CbEPSP – ''Coxiella burnetii''<br />
**[[3ti2]] - VcEPSP N terminal – ''Vibrio cholerae''<br />
**[[3rmt]] – EPSP – ''Bacillus halodurans''<br />
**[[5bs5]], [[5buf]] – EPSP – ''Acinetobacter baumannii''<br />
**[[7tm4]] - KpEPSP – ''Klebsiella pneumaniae''<br />
*EPSP synthase binary complex
**[[1g6t]] - EcEPSP + S3P <br />
**[[1mi4]], [[2qfq]], [[2qfs]], [[3fjx]], [[3fk0]] - EcEPSP (mutant) + S3P <br />
**[[1q36]] - EcEPSP (mutant) + S3P derivative<br />
**[[1x8r]], [[1x8t]] - EcEPSP + intermediate <br />
**[[2aa9]] – EcEPSP + shikimate<br />
**[[2pq9]] - EcEPSP + intermediate <br />
**[[1rf4]] - SpEPSP + intermediate <br />
**[[2o0b]], [[2o0d]] - MtEPSP + S3P <br />
**[[2gg6]] - AgEPSP + S3P <br />
**[[2pqb]], [[2pqc]], [[2pqd]] - AgEPSP + intermediate <br />
**[[2o0x]] - MtEPSP + intermediate <br />
**[[2o0z]] - MtEPSP + EPS <br />
**[[4egr]] - CbEPSP + phosphoenolpyruvate<br />
*EPSP synthase ternary complex
**[[1g6s]] - EcEPSP + S3P + glyphosate<br />
**[[2qft]], [[2qfu]], [[3fjz]], [[3fk1]] - EcEPSP (mutant) + S3P + glyphosate<br />
**[[2aay]] - EcEPSP + shikimate + glyphosate<br />
**[[1rf6]] - SpEPSP + S3P + glyphosate<br />
**[[2gga]] - AgEPSP + S3P + glyphosate<br />
**[[2ggd]] - AgEPSP (mutant) + S3P + glyphosate<br />
**[[2o0e]] - MtEPSP + S3P + phosphoenolpyruvate<br />
**[[3nvs]] - VcEPSP + S3P + glyphosate <br />
**[[3slh]] - CbEPSP + S3P + glyphosate<br />
}}
== References ==
== References ==
<references/>
<references/>


[[Category:Topic Page]]
[[Category:Topic Page]]

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Ann Taylor, Michal Harel, Alexander Berchansky, Joel L. Sussman
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