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| <StructureSection load='' size='350' side='right' caption='Structure of E. coli EPSP synthase complex with shikimate-3-phosphate, the herbicide glyphosate and formic acid (PDB entry [[1g6s]])' scene='57/570585/Cv/1'> | | <StructureSection load='' size='350' side='right' caption='Structure of E. coli EPSP synthase complex with shikimate-3-phosphate, the herbicide glyphosate and formic acid (PDB entry [[1g6s]])' scene='57/570585/Cv/1'> |
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| | __TOC__ |
| == Function == | | == Function == |
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| == Structural insights == | | == Structural insights == |
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| The enzyme has two domains, with the active site found in the interdomain cleft <scene name='57/570585/Two_domains/2'>(open conformation)</scene>. There is a substantial structural change upon substrate binding, resulting in a <scene name='57/570585/Closed_formation/2'>closed</scene> conformation. <scene name='57/570585/Cv/3'>See animation of this process</scene>. '''Glyphosate''' (also known as '''Roundup''') occupies the of the second substrate, phosphoenol pyruvate <ref>PMID:11171958</ref>. Interestingly CP4 EPSP synthase still binds glyphosate in the absence of PEP, but a conformational change in glyphosate to accomodate a steric clash with <scene name='57/570585/Glyphosate_s3p_distance/2'>Glu 354</scene> shortens the length of glyphosate, from 7.3 angstroms to 6.67 angstroms, and changes the IC50 by a factor of over 4,000, from 2.5 micromolar to 11 millimolar. | | The enzyme has two domains, with the active site found in the interdomain cleft <scene name='57/570585/Two_domains/5'>(open conformation)</scene>. There is a substantial structural change upon substrate binding, resulting in a <scene name='57/570585/Closed_formation/3'>closed</scene> conformation. <scene name='57/570585/Cv/9'>See animation of this process</scene>. '''Glyphosate''' (also known as '''Roundup''') occupies the <scene name='57/570585/Glyphosate_s3p/1'>binding site</scene> of the second substrate, phosphoenol pyruvate <ref>PMID:11171958</ref>. Interestingly CP4 EPSP synthase still binds glyphosate in the absence of PEP, but a conformational change in glyphosate to accommodate a steric clash with <scene name='57/570585/Glyphosate_s3p_distance/2'>Glu 354</scene> shortens the length of glyphosate, from 7.3 angstroms to 6.67 angstroms, and changes the IC50 by a factor of over 4,000, from 2.5 micromolar to 11 millimolar. |
| </StructureSection>
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| ===3D structures of EPSP synthase ===
| | ==3D structures of EPSP synthase == |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | [[EPSP synthase 3D structures]] |
| {{#tree:id=OrganizedByTopic|openlevels=0|
| | </StructureSection> |
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| *5-enolpyruvylshikimate 3-phosphate (EPSP) synthase
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| **[[1eps]] – EcEPSP – ''Escherichia coli''<br />
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| **[[1p88]], [[1p89]] - EcEPSP N terminal - NMR<br />
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| **[[1rf5]] - SpEPSP – ''Streptococcus pneumoniae''<br />
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| **[[2bjb]], [[2o15]] – MtEPSP – ''Mycobacterium tuberculosis''<br />
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| **[[2gg4]] – AgEPSP – ''Agrobacterium''<br />
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| **[[3roi]], [[3tr1]], [[4gfp]] – CbEPSP – ''Coxiella burnetii''<br />
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| **[[3ti2]] - VcEPSP N terminal – ''Vibrio cholerae''<br />
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| **[[3rmt]] – EPSP – ''Bacillus halodurans''<br />
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| *EPSP synthase binary complex
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| **[[1g6t]] - EcEPSP + S3P <br />
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| **[[1mi4]], [[2qfq]], [[2qfs]], [[3fjx]], [[3fk0]] - EcEPSP (mutant) + S3P <br />
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| **[[1q36]] - EcEPSP (mutant) + S3P derivative<br />
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| **[[1x8r]], [[1x8t]] - EcEPSP + intermediate <br />
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| **[[2aa9]] – EcEPSP + shikimate<br />
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| **[[2pq9]] - EcEPSP + intermediate <br />
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| **[[1rf4]] - SpEPSP + intermediate <br />
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| **[[2o0b]], [[2o0d]] - MtEPSP + S3P <br />
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| **[[2gg6]] - AgEPSP + S3P <br />
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| **[[2pqb]], [[2pqc]], [[2pqd]] - AgEPSP + intermediate <br />
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| **[[2o0x]] - MtEPSP + intermediate <br />
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| **[[2o0z]] - MtEPSP + EPS <br />
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| **[[4egr]] - CbEPSP + phosphoenolpyruvate<br />
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| *EPSP synthase ternary complex
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| **[[1g6s]] - EcEPSP + S3P + glyphosate<br />
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| **[[2qft]], [[2qfu]], [[3fjz]], [[3fk1]] - EcEPSP (mutant) + S3P + glyphosate<br />
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| **[[2aay]] - EcEPSP + shikimate + glyphosate<br />
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| **[[1rf6]] - SpEPSP + S3P + glyphosate<br />
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| **[[2gga]] - AgEPSP + S3P + glyphosate<br />
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| **[[2ggd]] - AgEPSP (mutant) + S3P + glyphosate<br />
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| **[[2o0e]] - MtEPSP + S3P + phosphoenolpyruvate<br />
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| **[[3nvs]] - VcEPSP + S3P + glyphosate <br />
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| **[[3slh]] - CbEPSP + S3P + glyphosate<br />
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
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| [[Category:Topic Page]] | | [[Category:Topic Page]] |