Titin: Difference between revisions

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Alexander Berchansky, David Canner, Michal Harel, Jaime Prilusky

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-[[Image:2rik.png|left|200px|thumb|Crystal Structure of I-band fragment I67-I69 from rabbit titin, [[2rik]]]]
+<StructureSection load="1ya5" size="350" caption="Human titin dimer residues 1-196 (grey and pale green) complex with telethonin (dark green) and sulfate, [[1ya5]]">
-{{STRUCTURE_2rik|  PDB=2rik  | SIZE=300| SCENE= |right|CAPTION=I-band fragment I67-I69 from rabbit titin, [[2rik]] }}
+[[Titin]] (TTN) is the largest known protein.  The human TTN contains 34,350 residues.  It is responsible for the passive elasticity of muscle.  It has 244 domains connected by unstructured regions.  The domains unfold when TTN is stretched. <scene name='41/417484/Cv/1'>Human titin dimer residues 1-196 complex with telethonin</scene> ([[1ya5]]). Additional details in<br />
+*[[Titin Structure & Function]]<br />
+*[[Group:MUZIC:Titin]].
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+==Introduction==
-[[Titin]] (TTN) is the largest known protein.  The human TTN contains 34,350 residues.  It is responsible for the passive elasticity of muscle.  It has 244 domains connected by unstructured regions.  The domains unfold when TTN is stretched. The images at the left and at the right correspond to one representative titin, ''i.e.'' the crystal structure of I-band fragment I67-I69 from rabbit titin ([[2rik]]).
+'''Titin''', also known as '''connectin''', is an elastic and approximately 3,6 MDalton large protein which assembles itself to protein filaments. It is made of more than 30000 amino acids and includes 320 protein domains and therefore is known as the largest human protein.
+It is a part of the [http://en.wikipedia.org/wiki/Sarcomere sarcomere], the smallest functional unit in the striated muscles. Tintins task in the sarcomere is to center the [http://en.wikipedia.org/wiki/Myosin myosinheads] between the [http://en.wikipedia.org/wiki/Actin actin] filaments and to re-establish the unstreched mode.
-{{TOC limit|limit=2}}
+The titin filament together with the actin filament is connected mechanically over the alpha-actinin with the z-disk. This bond is relatively weak and well regulateable, this is a necessary precondition for the unhindered contraction mechanism. Additionally titin binds to telethonin (t-cap) in the region of the z-disk. In the region of the A-band titin is accumulated with its stiff end near the myosin filament and binds to the calcium dependable protease [http://en.wikipedia.org/wiki/Calpain calpain].
+In the region of the I-band the long polypeptide becomes elastic and can be turned. The assembly of titin differs in the skeletal- and the cardiac muscle, whereby the marginal expandability of the cardiac sarcomer can be explained.
+The titin filament is not facilitated with the muscle contraction; it just participates in the flexibility and stability of the muscle and helps to define the contraction speed. In addition, titin is responsible for the relaxing mode of the muscle.
+[[Image:TITIN-Z1Z2 strechend unstreched.jpg|thumb|Z1Z2 stretched unstretched]]
+[[Image:titin-t.jpg|thumb|Titin scheme]]
+[[Image:Overview2-t.jpg|thumb|z1z2 / Telethonin complex]]
+Titin, the third most abundant and largest known protein with a lenght > 1 μm, is made of more than 300 tandem repeats of [http://de.wikipedia.org/wiki/Antikörper immunoglobulin-like (Ig) domains], [http://de.wikipedia.org/wiki/Fibronektin fibronectin type (FN-III)] repeats and from flexible [http://de.wikipedia.org/wiki/Random_Coil random coil]-like PEVK which are rich in [http://en.wikipedia.org/wiki/Amino_acid proline, glutamate, valine and lysine] regions. The insertion of single Lg-domains lead to isoforms of titin that can be found in the [http://en.wikipedia.org/wiki/Cardiac_muscle cardiac] and [http://en.wikipedia.org/wiki/Skeletal_muscle skeletal] muscles.
+The N-terminal part of titin interacts with some sarcomeric proteins at the Z-disc. These interactions maintain structural assistance for the development and function of [http://en.wikipedia.org/wiki/Myofibril myofibrils] and also have regulatory influence, such as responding to stretching forces developed in titin. Unique among them, is the structural protein [http://www.proteopedia.org/wiki/index.php/1ya5 telethonin], which is believed to anchor the ends of two separate titin molecules to the Z-disc. The C-terminal region is a part of the sarcomeric M-line.
+In picture (Z1Z2 stretched unstretched) one can see the highly schematic view of the tertiary structure elasticity of titin due to bending adjacent protein domains open and closed. In real titin, not every linker may contribute high flexibility, some linkers might be short and stiff.
+Extensive studies have shown that titin acts in many ways. Titin is believed to be a molecular scaffold in the early growth of myofibrill and that its end is not just an anchor, furthermore they interact with signaling proteins at the Z-line and M-line. It also functions as a molecular spring that dampens sarcomeric extension forces.
+The I-band region, that forms an elastic connection between the ends of thick filaments and the sarcomeric Z-disc, contains flexible PEVK domains interdigitated between Ig-domain repeats. These random coil PEVK domains are believed to reversibly unfold to permit the extension, like an entropic spring, and to contribute to titin elasticity when weak forces (50 pN) are applied.
+Titin has soft elasticity regions which are followed by stiffer, nonlinear regions of elasticity. The soft elasticity regions are a generic behavior of random coils formed through PEVK and similar domains and also because of straightening out of titin's multidomain segments. The stiffer elasticity is due to individual domains. Strong forces (160 pN) on titin unravels its secondary structure.
+Adjacent segments may exhibit a flexibility that leads a titin chain to assume in the relaxed state, a locally compacted shape and a straight shape when under tension.
+As seen in the image (Z1Z2 / Telethonin complex), the major force enduring component of this complex is an elaborate intermolecular hydrogen bonding network formed across <scene name='2a38/Test/2'>β-strand</scene> among telethonin and Z1Z2 domains, and not intramolecularly among termini β-strands of individual Z1 or Z2 domains. This shift to a stronger force enduring interface reduces the possibility of unraveling the individual Ig-domains, thus stabilizing the complex. This demonstrates how <scene name='2a38/Test/2'>β-strand</scene> cross-linking via [http://en.wikipedia.org/wiki/Hydrogen_bonds hydrogen bonds] serves as an important mechanism. It functions as a molecular adhesive, increasing the ability of protein complexes to resist against mechanical stress.
+==Disease==
+See [[Titin related diseases]].
+== 3D Structures of Titin ==
+[[Titin 3D structures]]
 ==Additional Resources==
 See: [[Titin Structure & Function]] for additional information <br />
+==See Also==
-== 3D Structures of Titin ==
+http://www.biophysik.physik.uni-muenchen.de/movies/movies-and-animations
+</StructureSection>
-[[2wp3]], [[2wwm]], [[3knb]] – hTTN residues 34252-34350+obscuring-like protein 1 fragment – human<br />
+==References==
-[[2wwk]] - hTTN residues 34252-34350+obscuring-like protein 1 fragment (mutant)<br />
+*http://www.ncbi.nlm.nih.gov:80/pmc/articles/PMC1948054/?tool=pmcentrez
-[[2rq8]] – hTTN residues 12677-12765 (mutant) – NMR<br />
+*http://www.ks.uiuc.edu/Research/z1z2/
-[[1tit]], [[1tiu]] - hTTN I27 – NMR<br />
+*http://www.ks.uiuc.edu/Research/telethonin/
-[[2rik]] – rTTN I67-I69 – rabbit<br />
+*http://de.wikipedia.org/wiki/Titin
-[[2rjm]] - rTTN I67-I69 (mutant)<br />
+[[Category:Topic Page]]
-[[3b43]] - rTTN I65-I70<br />
+[[Category: Homo sapiens]]
-[[2j8h]], [[2j8o]], [[2ill]] – hTTN  residues 24430-24623<br />
+[[Category: Demirel, M.]]
-[[2nzi]] – hTTN residues 31854-32155<br />
+[[Category: Marino, M.]]
-[[2bk8]] – hTTN residues 25073-25166<br />
+[[Category: Mayans, O.]]
-[[1waa]] - hTTN residues 12801-12889<br />
+[[Category: Muhle-Goll, C.]]
-[[2f8v]], [[1ya5]] - hTTN residues 1-196+telethonin<br />
+[[Category: Svergun, D.]]
-[[2a38]] - hTTN residues 1-194<br />
+[[Category: Titin]]
-[[1g1c]] - hTTN residues 2027-2125<br />
+[[Category: Z1z2]]
-[[1h8b]] – rTTN residues 648-698+h α Actinin 2 EF hands 3&4<br />
+<br />
-[[1bpv]] – hTTN A71 – NMR<br />
+Created with the participation of [[User:Anton Schmidt|Anton Schmidt]], [[User:Wolfgang Hermann|Wolfgang Hermann]].
-[[1tki]] – hTTN serine kinase fragment<br />
-[[1nct]], [[1ncu]] – hTTN M5 – NMR<br />
-[[1tnm]], [[1tnn]] – hTTN M5<br />