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| <StructureSection load='1khf' size='450' side='right' caption='GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry [[1khf]])' scene='54/540171/Cv/1'> | | <StructureSection load='' size='350' side='right' caption='GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry [[1khf]])' scene='54/540171/Cv/1'> |
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| == Function == | | == Function == |
| '''Phosphoenolpyruvate carboxykinase''' (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO<sub>2</sub>. PEPCK is part of the gluconeogenesis pathway<ref>PMID:9242918</ref>. There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate. | | '''Phosphoenolpyruvate carboxykinase''' (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO<sub>2</sub>. PEPCK is part of the [[gluconeogenesis]] pathway<ref>PMID:9242918</ref>. There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate. |
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| | See also: [[Gluconeogenesis]]. |
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| == Structural highlights == | | == Structural highlights == |
| ''E. coli'' GTP-driven PEPCK <scene name='54/540171/Cv/4'>active site</scene> is located in a pocket at the enzyme surface<ref>PMID:11851336</ref>. Water molecules shown as red spheres. | | ''E. coli'' GTP-driven PEPCK <scene name='54/540171/Cv/9'>active site</scene> is located in a pocket at the enzyme surface<ref>PMID:11851336</ref>. Water molecules are shown as red spheres. |
| *<scene name='54/540171/Cv/6'>Mn coordination site</scene>. | | *<scene name='54/540171/Cv/10'>Mn coordination site</scene>. |
| *<scene name='54/540171/Cv/8'>Na coordination site</scene>. | | *<scene name='54/540171/Cv/11'>Na coordination site</scene>. |
| | == 3D Structures of PEPCK == |
| | [[Phosphoenolpyruvate carboxykinase 3D structures]] |
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| </StructureSection> | | </StructureSection> |
| == 3D Structures of PEPCK ==
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *Phosphoenolpyruvate carboxykinase ATP driven
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| **[[1oen]], [[1fiy]] – EcPEPCK - ''Escherichia coli''<br />
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| **[[1ayl]] – EcPEPCK + ATP + oxalate + Mg<br />
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| **[[1aq2]] – EcPEPCK + ATP + pyruvate + Mg + Mn<br />
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| **[[2py7]] – EcPEPCK (mutant) + ATP + Mg + Mn<br />
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| **[[2olq]] – EcPEPCK + ATP + CO2 + Mg + Mn<br />
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| **[[2pxz]] – EcPEPCK + ATP + CO2 + oxaloacetate + Mg + Mn<br />
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| **[[2olr]] – EcPEPCK + ATP + CO2 + Mg <br />
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| **[[1os1]] – EcPEPCK + ATP + pyruvate + Mg + Ca<br />
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| **[[1k3c]] – EcPEPCK + ADP + pyruvate + Mg + AlF3<br />
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| **[[1k3d]] – EcPEPCK + ADP + Mg + AlF3<br />
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| **[[1j3b]] – TtPEPCK – ''Thermus thermophilus'' <br />
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| **[[1yvy]] – AnsPEPCK – ''Anaerobiospirillum succiniciproducens''<br />
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| **[[1ytm]] – AnsPEPCK + ATP + oxalate + Mg + Mn<br />
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| **[[1xkv]], [[2pc9]] – TtPEPCK + ATP <br />
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| *Phosphoenolpyruvate carboxykinase GTP driven
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| **[[1khf]] – EcPEPCK + PEP + Mn <br />
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| **[[1khg]] – EcPEPCK + Mn <br />
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| **[[1m51]], [[1nhx]] – EcPEPCK + inhibitor + Mn<br />
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| **[[2faf]] – cPEPCK + Mn - chicken<br />
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| **[[2qzy]] – cPEPCK + PEP + Mn <br />
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| **[[2gmv]] – hPEPCK + inhibitor + PEP + Mn - human<br />
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| **[[2qew]] – rPEPCK + Mn - rat<br />
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| **[[2qf1]] – rPEPCK + oxaloacetate + Mn<br />
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| **[[2rk7]] – rPEPCK + oxalate + Mn<br />
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| **[[2rk8]], [[2rka]], [[2rkd]], [[2rke]] – rPEPCK + inhibitor + Mn<br />
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| **[[4wiu]] – MtPEPCK + oxalate + Mn – ''Mycobacterium tuberculosis''<br />
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| **[[4wie]] – MtPEPCK + Mn <br />
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| *PEPCK GTP driven complex with nucleotide
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| **[[1khb]] – EcPEPCK + GTP analog + Mg <br />
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| **[[1khe]] – EcPEPCK + GTP analog + Mn <br />
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| **[[2fah]] – cPEPCK + GDP + Mn <br />
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| **[[2qey]] – rPEPCK + GTP + Mn<br />
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| **[[4gnm]], [[4gnq]] - rPEPCK + oxalate + GTP + Mn<br />
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| **[[4gmw]], [[4gnl]], [[4gnp]] – rPEPCK + PEP + GDP + Mn <br />
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| **[[3dt2]], [[3dt4]], [[4gmu]] – rPEPCK + GTP + oxalate + Mn<br />
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| **[[3mof]] – rPEPCK (mutant) + GTP + oxalate + Mn<br />
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| **[[3dt7]], [[4gmm]], [[4gmz]], [[4gno]] – rPEPCK + GTP + sulfopyruvate + Mn<br />
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| **[[3moe]], [[4ox2]] – rPEPCK (mutant) + GTP + sulfopyruvate + Mn<br />
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| **[[3dtb]] – rPEPCK + GTP + phosphoglycolate + Mn<br />
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| **[[3moh]] – rPEPCK (mutant) + GDP + phosphoglycolate + Mn<br />
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| **[[2qf2]] – rPEPCK + GDP + oxaloacetate + Mn<br />
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| **[[4rcg]] – MtPEPCK + GDP + Mg <br />
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| **[[4r43]] – MtPEPCK + GDP + Mn <br />
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| *PEPCK
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| **[[2zci]] – PEPCK – ''Corynebacterium glutamicum'' <br />
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| **[[1ygg]] – AsPEPCK – ''Actinobacillus succinogenes''<br />
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| **[[1ylh]] – AsPEPCK + pyruvate + Mn<br />
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| **[[1ii2]] – PEPCK – ''Trypanosoma cruzi''<br />
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |