Phosphoenolpyruvate carboxykinase: Difference between revisions

Latest revision as of 11:39, 10 January 2023

Function

Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO₂. PEPCK is part of the gluconeogenesis pathway^[1]. There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate.

Structural highlights

E. coli GTP-driven PEPCK is located in a pocket at the enzyme surface^[2]. Water molecules are shown as red spheres.

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3D Structures of PEPCK

Phosphoenolpyruvate carboxykinase 3D structures

GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry 1khf)

Drag the structure with the mouse to rotate

ReferencesReferences

↑ Hanson RW, Reshef L. Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression. Annu Rev Biochem. 1997;66:581-611. PMID:9242918 doi:http://dx.doi.org/10.1146/annurev.biochem.66.1.581
↑ Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ. Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site. J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:11851336 doi:http://dx.doi.org/10.1006/jmbi.2001.5364

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Michal Harel, Jaime Prilusky, Alexander Berchansky, Joel L. Sussman

[1] Hanson RW, Reshef L. Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression. Annu Rev Biochem. 1997;66:581-611. PMID:9242918 doi:http://dx.doi.org/10.1146/annurev.biochem.66.1.581

[2] Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ. Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site. J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:11851336 doi:http://dx.doi.org/10.1006/jmbi.2001.5364

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+<StructureSection load='' size='350' side='right' caption='GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry [[1khf]])' scene='54/540171/Cv/1'>
-[[Image:2olr.png|left|200px|thumb|Crystal structure of ATP-driven E. coli PEPCK complex with ATP, CO2 and Mg+2  [[2olr]]]]
+== Function ==
-{{STRUCTURE_2olr|  PDB=2olr  | SIZE=350| SCENE= |right|CAPTION=ATP-driven E. coli PEPCK complex with ATP, CO2 and Mg+2   [[2olr]] }}
+'''Phosphoenolpyruvate carboxykinase''' (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO<sub>2</sub>.  PEPCK is part of the [[gluconeogenesis]] pathway<ref>PMID:9242918</ref>.  There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate.
+See also: [[Gluconeogenesis]].
+== Structural highlights ==
+''E. coli'' GTP-driven PEPCK <scene name='54/540171/Cv/9'>active site</scene> is located in a pocket at the enzyme surface<ref>PMID:11851336</ref>. Water molecules are shown as red spheres.
+*<scene name='54/540171/Cv/10'>Mn coordination site</scene>.
+*<scene name='54/540171/Cv/11'>Na coordination site</scene>.
-'''Phosphoenolpyruvate carboxykinase''' (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO2.  PEPCK is part of the gluconeogenesis pathway.  There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate.
 == 3D Structures of PEPCK ==
+[[Phosphoenolpyruvate carboxykinase 3D structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-===PEPCK ATP driven===
-[[1oen]], [[1fiy]] – EcPEPCK - ''Escherichia coli''<br />
-[[1ayl]] – EcPEPCK + ATP + oxalate + Mg<br />
-[[1aq2]] – EcPEPCK + ATP + pyruvate + Mg + Mn<br />
-[[2py7]] – EcPEPCK (mutant) + ATP + Mg + Mn<br />
-[[2olq]] – EcPEPCK + ATP + CO2 + Mg + Mn<br />
-[[2pxz]] – EcPEPCK + ATP + CO2 + oxaloacetate + Mg + Mn<br />
-[[2olr]] – EcPEPCK + ATP + CO2 + Mg <br />
-[[1os1]] – EcPEPCK + ATP + pyruvate + Mg + Ca<br />
-[[1k3c]] – EcPEPCK + ADP + pyruvate + Mg + AlF3<br />
-[[1k3d]] – EcPEPCK + ADP + Mg + AlF3<br />
-[[1j3b]] – TtPEPCK – ''Thermus thermophilus'' <br />
-[[1yvy]] – AnsPEPCK – ''Anaerobiospirillum succiniciproducens''<br />
-[[1ytm]] – AnsPEPCK + ATP + oxalate + Mg + Mn<br />
-[[1xkv]], [[2pc9]] – TtPEPCK + ATP <br />
-===PEPCK GTP driven===
-[[1khb]] – EcPEPCK + GTP analog + Mg <br />
-[[1khe]] – EcPEPCK + GTP analog + Mn <br />
-[[1khf]] – EcPEPCK + PEP + Mn <br />
-[[1khg]] – EcPEPCK + Mn <br />
-[[1m51]], [[1nhx]] – EcPEPCK + inhibitor + Mn<br />
-[[2faf]] – cPEPCK + Mn - chicken<br />
-[[2fah]] – cPEPCK + GDP + Mn <br />
-[[2qzy]] – cPEPCK + PEP + Mn <br />
-[[2gmv]] – hPEPCK + inhibitor + PEP + Mn - human<br />
-[[2qew]] – rPEPCK + Mn - rat<br />
-[[2qey]] – rPEPCK + GTP + Mn<br />
-[[2qf1]] – rPEPCK + oxaloacetate + Mn<br />
-[[2rk7]] – rPEPCK + oxalate + Mn<br />
-[[3dt2]], [[3dt4]] – rPEPCK + GTP + oxalate + Mn<br />
-[[3mof]] – rPEPCK (mutant) + GTP + oxalate + Mn<br />
-[[3dt7]] – rPEPCK + GTP + sulfopyruvate + Mn<br />
-[[3moe]] – rPEPCK (mutant) + GTP + sulfopyruvate + Mn<br />
-[[3dtb]] – rPEPCK + GTP + phosphoglycolate + Mn<br />
-[[3moh]] – rPEPCK (mutant) + GDP + phosphoglycolate + Mn<br />
-[[2rk8]], [[2rka]], [[2rkd]], [[2rke]] – rPEPCK + inhibitor + Mn<br />
-[[2qf2]] – rPEPCK + GDP + oxaloacetate + Mn<br />
-[[2zci]] – PEPCK – ''Corynebacterium glutamicum'' <br />
-[[1ygg]] – AsPEPCK – ''Actinobacillus succinogenes''<br />
+== References ==
-[[1ylh]] – AsPEPCK + pyruvate + Mn<br />
+<references/>
+[[Category:Topic Page]]