1czq: Difference between revisions

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-[[Image:1czq.gif|left|200px]]<br />
-<applet load="1czq" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1czq, resolution 1.50&Aring;" />
-'''CRYSTAL STRUCTURE OF THE D10-P1/IQN17 COMPLEX: A D-PEPTIDE INHIBITOR OF HIV-1 ENTRY BOUND TO THE GP41 COILED-COIL POCKET.'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE D10-P1/IQN17 COMPLEX: A D-PEPTIDE INHIBITOR OF HIV-1 ENTRY BOUND TO THE GP41 COILED-COIL POCKET.==
-The HIV-1 gp41 protein promotes viral entry by mediating the fusion of, viral and cellular membranes. A prominent pocket on the surface of a, central trimeric coiled coil within gp41 was previously identified as a, potential target for drugs that inhibit HIV-1 entry. We designed a, peptide, IQN17, which properly presents this pocket. Utilizing IQN17 and, mirror-image phage display, we identified cyclic, D-peptide inhibitors of, HIV-1 infection that share a sequence motif. A 1.5 A cocrystal structure, of IQN17 in complex with a D-peptide, and NMR studies, show that conserved, residues of these inhibitors make intimate contact with the gp41 pocket., Our studies validate the pocket per se as a target for drug development., IQN17 and these D-peptide inhibitors are likely to be useful for, development and identification of a new class of orally bioavailable, anti-HIV drugs.
+<StructureSection load='1czq' size='340' side='right'caption='[[1czq]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1czq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CZQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CZQ FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DAR:D-ARGININE'>DAR</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DGL:D-GLUTAMIC+ACID'>DGL</scene>, <scene name='pdbligand=DHI:D-HISTIDINE'>DHI</scene>, <scene name='pdbligand=DLE:D-LEUCINE'>DLE</scene>, <scene name='pdbligand=DTR:D-TRYPTOPHAN'>DTR</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1czq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1czq OCA], [https://pdbe.org/1czq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1czq RCSB], [https://www.ebi.ac.uk/pdbsum/1czq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1czq ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The HIV-1 gp41 protein promotes viral entry by mediating the fusion of viral and cellular membranes. A prominent pocket on the surface of a central trimeric coiled coil within gp41 was previously identified as a potential target for drugs that inhibit HIV-1 entry. We designed a peptide, IQN17, which properly presents this pocket. Utilizing IQN17 and mirror-image phage display, we identified cyclic, D-peptide inhibitors of HIV-1 infection that share a sequence motif. A 1.5 A cocrystal structure of IQN17 in complex with a D-peptide, and NMR studies, show that conserved residues of these inhibitors make intimate contact with the gp41 pocket. Our studies validate the pocket per se as a target for drug development. IQN17 and these D-peptide inhibitors are likely to be useful for development and identification of a new class of orally bioavailable anti-HIV drugs.
-==About this Structure==
+Inhibiting HIV-1 entry: discovery of D-peptide inhibitors that target the gp41 coiled-coil pocket.,Eckert DM, Malashkevich VN, Hong LH, Carr PA, Kim PS Cell. 1999 Oct 1;99(1):103-15. PMID:10520998<ref>PMID:10520998</ref>
-CZQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_and_human_immunodeficiency_virus Saccharomyces cerevisiae and human immunodeficiency virus] with CL and ACE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CZQ OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Inhibiting HIV-1 entry: discovery of D-peptide inhibitors that target the gp41 coiled-coil pocket., Eckert DM, Malashkevich VN, Hong LH, Carr PA, Kim PS, Cell. 1999 Oct 1;99(1):103-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10520998 10520998]
+</div>
-[[Category: Saccharomyces cerevisiae and human immunodeficiency virus]]
+<div class="pdbe-citations 1czq" style="background-color:#fffaf0;"></div>
-[[Category: Single protein]]
+== References ==
-[[Category: Carr, P.A.]]
+<references/>
-[[Category: Eckert, D.M.]]
+__TOC__
-[[Category: Hong, L.H.]]
+</StructureSection>
-[[Category: Kim, P.S.]]
+[[Category: Human immunodeficiency virus]]
-[[Category: Malashkevich, V.N.]]
+[[Category: Large Structures]]
-[[Category: ACE]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: CL]]
+[[Category: Carr PA]]
-[[Category: coiled coil]]
+[[Category: Eckert DM]]
-[[Category: envelope glycoprotein]]
+[[Category: Hong LH]]
+[[Category: Kim PS]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 13:57:26 2007''
+[[Category: Malashkevich VN]]