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| {{STRUCTURE_1tyf| PDB=1tyf | SIZE=400| SCENE= |right|CAPTION=E. coli Clp protease, [[1tyf]] }}
| | <StructureSection load='1tyf' size='350' side='right' caption='E. coli Clp protease catalytic subunit (PDB entry [[1tyf]])' scene='50/508398/Cv/1'> |
| | == Function == |
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| | '''Clp protease''' (CLP) is a serine peptidase which hydrolyzes proteins in the presence of ATP and Mg+2 ion. CLP is found in mitochondria. CLP participates in degradation of misfolded proteins.<ref>PMID:2197275</ref><br /> |
| | For more details see <br /> |
| | * [[Clp protease]] |
| | * [[Zinc Fingers]] |
| | * [[Molecular Playground/ClpP]] |
| | * [[Molecular Playground/E. coli ClpP]]. |
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| '''Clp protease''' (CLP) is a serine peptidase which hydrolyzes proteins in the presence of ATP and Mg+2 ion. CLP is found in mitochondria. CLP participates in degradation of misfolded proteins. CLP is a heterodimer containing an ATP-binding regulatory subunit A and catalytic subunit P. For more details see [[Clp protease]]. | | '''Clp'''X or '''ATP-dependent Clp protease ATP-binding subunit ClpX''' is a molecular chaperone which alters the shape of DNA during bacteriophage μ transposition.<ref>PMID:21736903</ref> For details see [[Molecular Playground/Hexameric ClpX]] |
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| ==3D structures of Clp protease== | | == Structural highlights == |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | CLP is a heterodimer containing an ATP-binding regulatory subunit A '''ClpA''' or Hsp100 in [[Heat Shock Proteins]] and catalytic subunit P '''ClpP'''. The proteolytic complex is composed of <scene name='50/508398/Cv/2'>2 heptameric rings of ClpP</scene> flanked by hexameric rings of ClpA. For full proteolytic activity ClpP must associate with one of two related ATPase subunits: '''ClpA''' and '''ClpX''' or '''ATP-binding Clp protease ATP-binding subunit ClpX'''. |
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| ===CLP P subunit=== | | <scene name='45/458468/Cv/4'>ADP binding site</scene> in ''Helicobacter pylori'' ClpX (PDB entry [[1um8]]).<ref>PMID:14514695</ref> |
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| [[1tyf]], [[1yg6]] – EcCLP – ''Escherichia coli''<br />
| | ==3D structures of Clp protease== |
| [[1yg8]], [[3hln]] - EcCLP (mutant)<br />
| | [[Clp protease 3D structures]] |
| [[1y7o]] – CLP – ''Streptococcus pneumoniae''<br />
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| [[1tg6]] - CLP – human<br />
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| [[2f6i]], [[4gm2]], [[4hnk]] - CLP – ''Plasmodium falciparum''<br />
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| [[2cby]], [[2ce3]], [[2c8t]] - CLP – ''Mycobacterium tuberculosis''<br />
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| [[3fes]] - CLP – ''Clostridium difficile''<br />
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| [[3ktg]], [[3kth]], [[3tt6]] – BsCLP – ''Bacillus subtilis''<br />
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| [[3p2l]] - CLP – ''Francisella tulerensis''<br />
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| [[3q7h]] - CLP – ''Coxiella burnetii''<br />
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| [[3qwd]], [[3st9]], [[3sta]], [[3v5e]], [[4emm]], [[4mxi]] - SaCLP – ''Staphylococcus aureus''<br />
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| [[3v5i]], [[4emp]] - SaCLP (mutant)<br />
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| [[2zl0]] - HpCLP – ''Helicobacter pylori''<br />
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| [[2zl3]] - HpCLP (mutant)<br />
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| [[4jcq]], [[4jct]] – LmCLP – ''Listeria monocytogenes''<br />
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| [[4jcr]] - LmCLP (mutant)<br />
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| ''CLP P subunit binary complex''
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| [[2fzs]], [[3mt6]] - EcCLP + peptide<br />
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| [[2zl2]] - HpCLP + peptide<br />
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| [[2zl4]] - HpCLP (mutant) + peptide<br />
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| [[3kti]], [[3ktj]], [[3ktk]] - BsCLP + peptide<br />
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| [[3tt7]] - BsCLP + DFP
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| ===CLP A subunit===
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| [[1k6k]], [[1r6c]] – EcCLP N terminal<br />
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| [[1ovx]] - EcCLP N terminal - NMR<br />
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| [[1ksf]], [[1r6b]] – EcCLP (mutant)<br />
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| [[1um8]] – HpCLP
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| ''CLP A subunit binary complex''
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| [[1lzw]], [[1mg9]], [[1mbu]], [[1mbv]], [[1mbx]] – EcCLP + CLP adaptor protein ClpS<br />
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| [[1r6o]], [[1r6q]] - EcCLP N terminal + CLP adaptor protein ClpS
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| ===ATP-dependent CLP - CLPX===
| | </StructureSection> |
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| [[2ds5]], [[2ds6]] – EcCLPX Zn-binding domain<br />
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| [[2ds7]] – EcCLPX Zn-binding domain (mutant)<br />
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| [[2ds8]] – EcCLPX Zn-binding domain + SSPB-tail peptide<br />
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| [[3hte]], [[4i34]], [[4i4l]], [[4i5o]], [[4i63]], [[4i9k]] – EcCLPX<br />
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| [[3hws]] – EcCLPX (mutant)<br />
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| [[4i81]] – EcCLPX + ATP-γ-S<br />
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| | == References == |
| | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |