Clp Protease: Difference between revisions

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Michal Harel, Alexander Berchansky

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-{{STRUCTURE_2zl2|  PDB=2zl2  | SIZE=400| SCENE= |right|CAPTION=Clp protease A subunit complex with peptide, [[2zl2]] }}
+<StructureSection load='1tyf' size='350' side='right' caption='E. coli Clp protease catalytic subunit (PDB entry [[1tyf]])' scene='50/508398/Cv/1'>
+== Function ==
-'''Clp protease''' (CLP) is a serine peptidase which hydrolyzes proteins in the presence of ATP and Mg+2 ion.  CLP is found in mitochondria.  CLP participates in degradation of misfolded proteins.  CLP is a heterodimer containing an ATP-binding regulatory subunit A and catalytic subunit P.  For more details see [[Clp protease]].
+'''Clp protease''' (CLP) is a serine peptidase which hydrolyzes proteins in the presence of ATP and Mg+2 ion.  CLP is found in mitochondria.  CLP participates in degradation of misfolded proteins.<ref>PMID:2197275</ref><br />
+For more details see <br />
+* [[Clp protease]]
+* [[Zinc Fingers]]
+* [[Molecular Playground/ClpP]]
+* [[Molecular Playground/E. coli ClpP]].
-==3D structures of Clp protease==
+'''Clp'''X or '''ATP-dependent Clp protease ATP-binding subunit ClpX''' is a molecular chaperone which alters the shape of DNA during bacteriophage μ transposition.<ref>PMID:21736903</ref>  For details see [[Molecular Playground/Hexameric ClpX]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+== Structural highlights ==
-===CLP P subunit===
+CLP is a heterodimer containing an ATP-binding regulatory subunit A '''ClpA''' or Hsp100 in [[Heat Shock Proteins]] and catalytic subunit P '''ClpP'''.  The proteolytic complex is composed of <scene name='50/508398/Cv/2'>2 heptameric rings of ClpP</scene> flanked by hexameric rings of ClpA.  For full proteolytic activity ClpP must associate with one of two related ATPase subunits: '''ClpA''' and '''ClpX''' or '''ATP-binding Clp protease ATP-binding subunit ClpX'''.
-[[1tyf]], [[1yg6]] – EcCLP – ''Escherichia coli''<br />
+<scene name='45/458468/Cv/4'>ADP binding site</scene> in ''Helicobacter pylori'' ClpX (PDB entry [[1um8]]).<ref>PMID:14514695</ref>
-[[1yg8]], [[3hln]] - EcCLP (mutant)<br />
-[[1y7o]] – CLP – ''Streptococcus pneumoniae''<br />
-[[1tg6]] - CLP – human<br />
-[[2f6i]] - CLP – ''Plasmodium falciparum''<br />
-[[2cby]], [[2ce3]], [[2c8t]] - CLP – ''Mycobacterium tuberculosis''<br />
-[[3fes]] - CLP – ''Clostridium difficile''<br />
-[[3ktg]], [[3kth]], [[3tt6]] – BsCLP – ''Bacillus subtilis''<br />
-[[3p2l]] - CLP – ''Francisella tulerensis''<br />
-[[3q7h]] - CLP – ''Coxiella burnetii''<br />
-[[3qwd]], [[3st9]], [[3sta]], [[3v5e]] - SaCLP – ''Staphylococcus aureus''<br />
-[[3v5i]] - SaCLP (mutant)<br />
-[[2zl0]] - HpCLP – ''Helicobacter pylori''<br />
-[[2zl3]] - HpCLP (mutant)<br />
-''CLP P subunit binary complex''
+==3D structures of Clp protease==
+[[Clp protease 3D structures]]
-[[2fzs]], [[3mt6]] - EcCLP + peptide<br />
-[[2zl2]] - HpCLP + peptide<br />
-[[2zl4]] - HpCLP (mutant) + peptide<br />
-[[3kti]], [[3ktj]], [[3ktk]] - BsCLP + peptide<br />
-[[3tt7]] - BsCLP + DFP
-===CLP A subunit===
-[[1k6k]], [[1r6c]] – EcCLP N terminal<br />
+</StructureSection>
-[[1ovx]] - EcCLP N terminal - NMR<br />
-[[1ksf]], [[1r6b]] – EcCLP (mutant)<br />
-[[1um8]] – HpCLP
-''CLP A subunit binary complex''
-[[1lzw]], [[1mg9]], [[1mbu]], [[1mbv]], [[1mbx]] – EcCLP + CLP adaptor protein ClpS<br />
+== References ==
-[[1r6o]], [[1r6q]] - EcCLP N terminal + CLP adaptor protein ClpS
+<references/>
 [[Category:Topic Page]]