7pip: Difference between revisions
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==70S ribosome with EF-Tu-tRNA and P-site tRNA in pseudouridimycin-treated Mycoplasma pneumoniae cells== | |||
<StructureSection load='7pip' size='340' side='right'caption='[[7pip]], [[Resolution|resolution]] 9.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7pip]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycoplasma_pneumoniae_M129 Mycoplasma pneumoniae M129]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PIP FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pip OCA], [https://pdbe.org/7pip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pip RCSB], [https://www.ebi.ac.uk/pdbsum/7pip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pip ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RL34_MYCPN RL34_MYCPN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Translation is the fundamental process of protein synthesis and is catalysed by the ribosome in all living cells(1). Here we use advances in cryo-electron tomography and sub-tomogram analysis(2,3) to visualize the structural dynamics of translation inside the bacterium Mycoplasma pneumoniae. To interpret the functional states in detail, we first obtain a high-resolution in-cell average map of all translating ribosomes and build an atomic model for the M. pneumoniae ribosome that reveals distinct extensions of ribosomal proteins. Classification then resolves 13 ribosome states that differ in their conformation and composition. These recapitulate major states that were previously resolved in vitro, and reflect intermediates during active translation. On the basis of these states, we animate translation elongation inside native cells and show how antibiotics reshape the cellular translation landscapes. During translation elongation, ribosomes often assemble in defined three-dimensional arrangements to form polysomes(4). By mapping the intracellular organization of translating ribosomes, we show that their association into polysomes involves a local coordination mechanism that is mediated by the ribosomal protein L9. We propose that an extended conformation of L9 within polysomes mitigates collisions to facilitate translation fidelity. Our work thus demonstrates the feasibility of visualizing molecular processes at atomic detail inside cells. | |||
Visualizing translation dynamics at atomic detail inside a bacterial cell.,Xue L, Lenz S, Zimmermann-Kogadeeva M, Tegunov D, Cramer P, Bork P, Rappsilber J, Mahamid J Nature. 2022 Oct;610(7930):205-211. doi: 10.1038/s41586-022-05255-2. Epub 2022, Sep 28. PMID:36171285<ref>PMID:36171285</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7pip" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mycoplasma pneumoniae M129]] | |||
[[Category: Lenz S]] | |||
[[Category: Mahamid J]] | |||
[[Category: Rappsilber J]] | |||
[[Category: Xue L]] | |||
Latest revision as of 22:31, 19 October 2022
70S ribosome with EF-Tu-tRNA and P-site tRNA in pseudouridimycin-treated Mycoplasma pneumoniae cells70S ribosome with EF-Tu-tRNA and P-site tRNA in pseudouridimycin-treated Mycoplasma pneumoniae cells
Structural highlights
FunctionPublication Abstract from PubMedTranslation is the fundamental process of protein synthesis and is catalysed by the ribosome in all living cells(1). Here we use advances in cryo-electron tomography and sub-tomogram analysis(2,3) to visualize the structural dynamics of translation inside the bacterium Mycoplasma pneumoniae. To interpret the functional states in detail, we first obtain a high-resolution in-cell average map of all translating ribosomes and build an atomic model for the M. pneumoniae ribosome that reveals distinct extensions of ribosomal proteins. Classification then resolves 13 ribosome states that differ in their conformation and composition. These recapitulate major states that were previously resolved in vitro, and reflect intermediates during active translation. On the basis of these states, we animate translation elongation inside native cells and show how antibiotics reshape the cellular translation landscapes. During translation elongation, ribosomes often assemble in defined three-dimensional arrangements to form polysomes(4). By mapping the intracellular organization of translating ribosomes, we show that their association into polysomes involves a local coordination mechanism that is mediated by the ribosomal protein L9. We propose that an extended conformation of L9 within polysomes mitigates collisions to facilitate translation fidelity. Our work thus demonstrates the feasibility of visualizing molecular processes at atomic detail inside cells. Visualizing translation dynamics at atomic detail inside a bacterial cell.,Xue L, Lenz S, Zimmermann-Kogadeeva M, Tegunov D, Cramer P, Bork P, Rappsilber J, Mahamid J Nature. 2022 Oct;610(7930):205-211. doi: 10.1038/s41586-022-05255-2. Epub 2022, Sep 28. PMID:36171285[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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