7vyq: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 7vyq is ON HOLD
+==Short chain dehydrogenase (SCR) cryoEM structure with NADP and ethyl 4-chloroacetoacetate==
+<StructureSection load='7vyq' size='340' side='right'caption='[[7vyq]], [[Resolution|resolution]] 3.13&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7vyq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VYQ FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=83I:ethyl+4-chloranyl-3-oxidanylidene-butanoate'>83I</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vyq OCA], [https://pdbe.org/7vyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vyq RCSB], [https://www.ebi.ac.uk/pdbsum/7vyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vyq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[https://www.uniprot.org/uniprot/B2KJ46_CANPA B2KJ46_CANPA]]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The evolutionary benefit accounting for widespread conservation of oligomeric structures in proteins lacking evidence of intersubunit cooperativity remains unclear. Here, crystal and cryo-EM structures, and enzymological data, demonstrate that a conserved tetramer interface maintains the active-site structure in one such class of proteins, the short-chain dehydrogenase/reductase (SDR) superfamily. Phylogenetic comparisons support a significantly longer polypeptide being required to maintain an equivalent active-site structure in the context of a single subunit. Oligomerization therefore enhances evolutionary fitness by reducing the metabolic cost of enzyme biosynthesis. The large surface area of the structure-stabilizing oligomeric interface yields a synergistic gain in fitness by increasing tolerance to activity-enhancing yet destabilizing mutations. We demonstrate that two paralogous SDR superfamily enzymes with different specificities can form mixed heterotetramers that combine their individual enzymological properties. This suggests that oligomerization can also diversify the functions generated by a given metabolic investment, enhancing the fitness advantage provided by this architectural strategy.
-Authors: Li, Y.H., Zhang, R.Z., Wang, C., Forouhar, F., Clarke, O., Vorobiev, S., Singh, S., Montelione, G.T., Szyperski, T., Xu, Y., Hunt, J.F.
+Oligomeric interactions maintain active-site structure in a noncooperative enzyme family.,Li Y, Zhang R, Wang C, Forouhar F, Clarke OB, Vorobiev S, Singh S, Montelione GT, Szyperski T, Xu Y, Hunt JF EMBO J. 2022 Jul 8:e108368. doi: 10.15252/embj.2021108368. PMID:35801308<ref>PMID:35801308</ref>
-Description: Oligomeric interactions maintain active-site structure in a non-cooperative enzyme family
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Vorobiev, S]]
+<div class="pdbe-citations 7vyq" style="background-color:#fffaf0;"></div>
-[[Category: Forouhar, F]]
+== References ==
-[[Category: Szyperski, T]]
+<references/>
-[[Category: Hunt, J.F]]
+__TOC__
-[[Category: Zhang, R.Z]]
+</StructureSection>
-[[Category: Xu, Y]]
+[[Category: Candida parapsilosis]]
-[[Category: Singh, S]]
+[[Category: Large Structures]]
-[[Category: Wang, C]]
+[[Category: Clarke O]]
-[[Category: Montelione, G.T]]
+[[Category: Forouhar F]]
-[[Category: Li, Y.H]]
+[[Category: Hunt JF]]
-[[Category: Clarke, O]]
+[[Category: Li YH]]
+[[Category: Montelione GT]]
+[[Category: Singh S]]
+[[Category: Szyperski T]]
+[[Category: Vorobiev S]]
+[[Category: Wang C]]
+[[Category: Xu Y]]
+[[Category: Zhang RZ]]