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| ==Crystal structure of the SepCysS-SepCysE N-terminal domain complex from== | | ==Crystal structure of the SepCysS-SepCysE N-terminal domain complex from== |
| <StructureSection load='3wks' size='340' side='right' caption='[[3wks]], [[Resolution|resolution]] 3.03Å' scene=''> | | <StructureSection load='3wks' size='340' side='right'caption='[[3wks]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3wks]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WKS FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WKS FirstGlance]. <br> |
| </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wks OCA], [https://pdbe.org/3wks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wks RCSB], [https://www.ebi.ac.uk/pdbsum/3wks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wks ProSAT]</span></td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wkr|3wkr]]</td></tr>
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| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/O-phospho-L-seryl-tRNA:Cys-tRNA_synthase O-phospho-L-seryl-tRNA:Cys-tRNA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.73 2.5.1.73] </span></td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wks OCA], [http://pdbe.org/3wks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wks RCSB], [http://www.ebi.ac.uk/pdbsum/3wks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wks ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function ==
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| [[http://www.uniprot.org/uniprot/SPSS_METJA SPSS_METJA]] Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).<ref>PMID:15790858</ref>
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| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Methanogenic archaea lack cysteinyl-tRNA synthetase; they synthesize Cys-tRNA and cysteine in a tRNA-dependent manner. Two enzymes are required: Phosphoseryl-tRNA synthetase (SepRS) forms phosphoseryl-tRNA(Cys) (Sep-tRNA(Cys)), which is converted to Cys-tRNA(Cys) by Sep-tRNA:Cys-tRNA synthase (SepCysS). This represents the ancestral pathway of Cys biosynthesis and coding in archaea. Here we report a translation factor, SepCysE, essential for methanococcal Cys biosynthesis; its deletion in Methanococcus maripaludis causes Cys auxotrophy. SepCysE acts as a scaffold for SepRS and SepCysS to form a stable high-affinity complex for tRNA(Cys) causing a 14-fold increase in the initial rate of Cys-tRNA(Cys) formation. Based on our crystal structure (2.8-A resolution) of a SepCysSSepCysE complex, a SepRSSepCysESepCysS structure model suggests that this ternary complex enables substrate channeling of Sep-tRNA(Cys). A phylogenetic analysis suggests coevolution of SepCysE with SepRS and SepCysS in the last universal common ancestral state. Our findings suggest that the tRNA-dependent Cys biosynthesis proceeds in a multienzyme complex without release of the intermediate and this mechanism may have facilitated the addition of Cys to the genetic code.
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| Ancient translation factor is essential for tRNA-dependent cysteine biosynthesis in methanogenic archaea.,Liu Y, Nakamura A, Nakazawa Y, Asano N, Ford KA, Hohn MJ, Tanaka I, Yao M, Soll D Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10520-5. doi:, 10.1073/pnas.1411267111. Epub 2014 Jul 7. PMID:25002468<ref>PMID:25002468</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3wks" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: O-phospho-L-seryl-tRNA:Cys-tRNA synthase]] | | [[Category: Large Structures]] |
| [[Category: Asano, N]] | | [[Category: Asano N]] |
| [[Category: Nakamura, A]] | | [[Category: Nakamura A]] |
| [[Category: Nakazawa, Y]] | | [[Category: Nakazawa Y]] |
| [[Category: Yao, M]] | | [[Category: Yao M]] |
| [[Category: Aminoacyl trna synthesis]]
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| [[Category: Transferase]]
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