4amq: Difference between revisions
New page: '''Unreleased structure''' The entry 4amq is ON HOLD until Paper Publication Authors: Lartigue, A., Claverie, J.M., Priet, S., Abergel, C. Description: A Megaviridae Orfan gene encodes... |
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==A Megaviridae Orfan gene encodes a new nucleotidyl transferase== | |||
<StructureSection load='4amq' size='340' side='right'caption='[[4amq]], [[Resolution|resolution]] 2.17Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4amq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Apmv Apmv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AMQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AMQ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4amq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4amq OCA], [https://pdbe.org/4amq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4amq RCSB], [https://www.ebi.ac.uk/pdbsum/4amq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4amq ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mimivirus is the prototype of a new family (the Mimiviridae) of nucleocytoplasmic large DNA viruses (NCLDVs), which already include the Poxviridae, Iridoviridae, Phycodnaviridae and Asfarviridae. Mimivirus specifically replicates in cells from the genus Acanthamoeba. Proteomic analysis of purified mimivirus particles revealed the presence of many subunits of the DNA-directed RNA polymerase II complex. A fully functional pre-transcriptional complex appears to be loaded in the virions, allowing mimivirus to initiate transcription within the host cytoplasm immediately upon infection independently of the host nuclear apparatus. To fully understand this process, a systematic study of mimivirus proteins that are predicted (by bioinformatics) or suspected (by proteomic analysis) to be involved in transcription was initiated by cloning and expressing them in Escherichia coli in order to determine their three-dimensional structures. Here, preliminary crystallographic analysis of the recombinant L544 protein is reported. The crystals belonged to the orthorhombic space group C222(1) with one monomer per asymmetric unit. A MAD data set was used for preliminary phasing using the selenium signal present in a selenomethionine-substituted protein crystal. | |||
Preliminary crystallographic analysis of a possible transcription factor encoded by the mimivirus L544 gene.,Ciaccafava A, Lartigue A, Mansuelle P, Jeudy S, Abergel C Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):922-5. doi:, 10.1107/S1744309111020847. Epub 2011 Jul 20. PMID:21821896<ref>PMID:21821896</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4amq" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Apmv]] | |||
[[Category: Large Structures]] | |||
[[Category: Abergel, C]] | |||
[[Category: Claverie, J M]] | |||
[[Category: Lartigue, A]] | |||
[[Category: Priet, S]] | |||
[[Category: Mg662]] | |||
[[Category: Mimivirus]] | |||
[[Category: Transcription coupled dna repair]] | |||
[[Category: Transferase]] | |||
Latest revision as of 08:39, 25 August 2022
A Megaviridae Orfan gene encodes a new nucleotidyl transferaseA Megaviridae Orfan gene encodes a new nucleotidyl transferase
Structural highlights
Publication Abstract from PubMedMimivirus is the prototype of a new family (the Mimiviridae) of nucleocytoplasmic large DNA viruses (NCLDVs), which already include the Poxviridae, Iridoviridae, Phycodnaviridae and Asfarviridae. Mimivirus specifically replicates in cells from the genus Acanthamoeba. Proteomic analysis of purified mimivirus particles revealed the presence of many subunits of the DNA-directed RNA polymerase II complex. A fully functional pre-transcriptional complex appears to be loaded in the virions, allowing mimivirus to initiate transcription within the host cytoplasm immediately upon infection independently of the host nuclear apparatus. To fully understand this process, a systematic study of mimivirus proteins that are predicted (by bioinformatics) or suspected (by proteomic analysis) to be involved in transcription was initiated by cloning and expressing them in Escherichia coli in order to determine their three-dimensional structures. Here, preliminary crystallographic analysis of the recombinant L544 protein is reported. The crystals belonged to the orthorhombic space group C222(1) with one monomer per asymmetric unit. A MAD data set was used for preliminary phasing using the selenium signal present in a selenomethionine-substituted protein crystal. Preliminary crystallographic analysis of a possible transcription factor encoded by the mimivirus L544 gene.,Ciaccafava A, Lartigue A, Mansuelle P, Jeudy S, Abergel C Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):922-5. doi:, 10.1107/S1744309111020847. Epub 2011 Jul 20. PMID:21821896[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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