3rwt: Difference between revisions

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-[[Image:3rwt.png|left|200px]]
-<!--
+==Crystal structure of circular permutated Red Fluorescent Protein mKate(cp 154-153)==
-The line below this paragraph, containing "STRUCTURE_3rwt", creates the "Structure Box" on the page.
+<StructureSection load='3rwt' size='340' side='right'caption='[[3rwt]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3rwt]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bubble-tip_anemone Bubble-tip anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RWT FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
--->
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
-{{STRUCTURE_3rwt|  PDB=3rwt  |  SCENE=  }}
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rwa|3rwa]]</div></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rwt OCA], [https://pdbe.org/3rwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rwt RCSB], [https://www.ebi.ac.uk/pdbsum/3rwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rwt ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Circular permutation of fluorescent proteins provides a substrate for the design of molecular sensors. Here we describe a systematic exploration of permutation sites for mCherry and mKate using a tandem fusion template approach. Circular permutants retaining more than 60% (mCherry) and 90% (mKate) brightness of the parent molecules are reported, as well as a quantitative evaluation of the fluorescence from neighboring mutations. Truncations of circular permutants indicated essential N- and C-terminal segments and substantial flexibility in the use of these molecules. Structural evaluation of two cp-mKate variants indicated no major conformational changes from the previously reported wild-type structure, and cis conformation of the chromophores. Four cp-mKates were identified with over 80% of native fluorescence, providing important new building blocks for sensor and complementation experiments.
-===Crystal structure of circular permutated Red Fluorescent Protein mKate(cp 154-153)===
+Circular permutation of red fluorescent proteins.,Shui B, Wang Q, Lee F, Byrnes LJ, Chudakov DM, Lukyanov SA, Sondermann H, Kotlikoff MI PLoS One. 2011;6(5):e20505. Epub 2011 May 27. PMID:21647365<ref>PMID:21647365</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3rwt" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_21647365}}, adds the Publication Abstract to the page
+*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 21647365 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_21647365}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Bubble-tip anemone]]
-[[3rwt]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RWT OCA].
+[[Category: Large Structures]]
+[[Category: Sondermann, H]]
-==Reference==
+[[Category: Wang, Q]]
-<ref group="xtra">PMID:021647365</ref><references group="xtra"/>
-[[Category: Entacmaea quadricolor]]
-[[Category: Sondermann, H.]]
-[[Category: Wang, Q.]]
 [[Category: Circular permutated]]
 [[Category: Fluorescent protein]]