Uridylate kinase: Difference between revisions
Michal Harel (talk | contribs) No edit summary |
Michal Harel (talk | contribs) No edit summary |
||
| (8 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
<StructureSection load='2j4k' size=' | <StructureSection load='2j4k' size='350' side='right' caption='Uridylate kinase hexamer complex with ligand UMP, Cd+ (gold) and Mg+2 (green) ions [[2j4k]]' scene='48/487531/Cv/1' > | ||
== Function == | == Function == | ||
'''Uridylate kinase''' (UK) catalyzes the reversible transfer of phosphate from UMP to UDP using ATP as a phosphate source. UDP is the starting point of synthesis of all other pyrimidine nucleotides. The eukaryotic UK has specificity to both UMP and CMP while the bacterial one is specific for UMP. The bacterial UK is Mg+2 dependent and is activated by GTP and repressed by UTP. The bacterial UK is composed of a C terminal ATP-binding domain and an N terminal UMP-binding domain<ref>PMID:10218107</ref>. | '''Uridylate kinase''' (UK) catalyzes the reversible transfer of phosphate from UMP to UDP using ATP as a phosphate source. UDP is the starting point of synthesis of all other pyrimidine nucleotides. The eukaryotic UK has specificity to both UMP and CMP while the bacterial one is specific for UMP. The bacterial UK is Mg+2 dependent and is activated by GTP and repressed by UTP. The bacterial UK is composed of a C terminal ATP-binding domain and an N terminal UMP-binding domain<ref>PMID:10218107</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
The active site of UK has high specificity to uracil and excludes purine nucleotides<ref>PMID:17297917</ref>. | The biological assembly of Uridylate kinase from ''Sulfolobus solfataricus'' ([[2j4k]]) is <scene name='48/487531/Cv/4'>homohexamer</scene>. The <scene name='48/487531/Cv/5'>active site of UK has high specificity to uracil</scene> and excludes purine nucleotides<ref>PMID:17297917</ref>. Water molecule is shown as red sphere. | ||
</StructureSection> | </StructureSection> | ||
==3D structures of uridylate kinase== | ==3D structures of uridylate kinase== | ||
| Line 23: | Line 23: | ||
*Uridylate kinase complex with nucleotides | *Uridylate kinase complex with nucleotides | ||
**[[1uky]] – yUK + ADP – yeast<br /> | |||
**[[1ukz]] – yUK + AMP + ADP<br /> | **[[1ukz]] – yUK + AMP + ADP<br /> | ||
**[[2bne]] – EcUK (mutant) + UMP – ''Escherichia coli''<br /> | **[[2bne]] – EcUK (mutant) + UMP – ''Escherichia coli''<br /> | ||
| Line 35: | Line 36: | ||
**[[2j4l]] - SsUK + UTP<br /> | **[[2j4l]] - SsUK + UTP<br /> | ||
**[[2jjx]] - UK + ATP + Mg – ''Bacillus anthracis''<br /> | **[[2jjx]] - UK + ATP + Mg – ''Bacillus anthracis''<br /> | ||
**[[3ek5]] - XcUK + GTP <br /> | **[[3ek5]] - XcUK + GTP <br /> | ||
**[[3nwy]] – | **[[3nwy]] – MtUK + UTP + GTP – ''Mycobacterium tuberculosis''<br /> | ||
**[[7bex]], [[7bix]], [[7bl7]] - MtUK + UTP + UDP<br /> | |||
**[[4a7x]], [[4a7w]] - UK + UDP – ''Helicobacter pylori''<br /> | **[[4a7x]], [[4a7w]] - UK + UDP – ''Helicobacter pylori''<br /> | ||
}} | }} | ||