Uridylate kinase: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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+<StructureSection load='2j4k' size='350' side='right' caption='Uridylate kinase hexamer complex with ligand UMP, Cd+ (gold)  and Mg+2 (green) ions [[2j4k]]' scene='48/487531/Cv/1' >
+== Function ==
+'''Uridylate kinase''' (UK) catalyzes the reversible transfer of phosphate from UMP to UDP using ATP as a phosphate source.  UDP is the starting point of synthesis of all other pyrimidine nucleotides.  The eukaryotic UK has specificity to both UMP and CMP while the bacterial one is specific for UMP.  The bacterial UK is Mg+2 dependent and is activated by GTP and repressed by UTP.  The bacterial UK is composed of a C terminal ATP-binding domain and an N terminal UMP-binding domain<ref>PMID:10218107</ref>.
-<StructureSection load='2j4k' size='340' side='right' caption='Uridylate kinase hexamer complex with ligand UMP (stick model), Cd+ (gold)  and Mg+2 (green) ions [[2j4k]]' scene='' >
+== Structural highlights ==
-<!--
+The biological assembly of Uridylate kinase from ''Sulfolobus solfataricus'' ([[2j4k]]) is <scene name='48/487531/Cv/4'>homohexamer</scene>. The <scene name='48/487531/Cv/5'>active site of UK has high specificity to uracil</scene> and excludes purine nucleotides<ref>PMID:17297917</ref>. Water molecule is shown as red sphere.
-Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page.
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--->
-'''Uridylate kinase''' (UK) catalyzes the reversible transfer of phosphate from UMP to UDP using ATP as a phosphate source.  UDP is the starting point of synthesis of all other pyrimidine nucleotides.  The eukaryotic UK has specificity to both UMP and CMP while the bacterial one is specific for UMP.  The bacterial UK is Mg+2 dependent and is activated by GTP and repressed by UTP.  The bacterial UK is composed of a C terminal ATP-binding domain and an N terminal UMP-binding domain<ref>PMID:15454079</ref>.
 </StructureSection>
 ==3D structures of uridylate kinase==
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 *Uridylate kinase complex with nucleotides
+**[[1uky]] – yUK + ADP – yeast<br />
 **[[1ukz]] – yUK + AMP + ADP<br />
 **[[2bne]] – EcUK (mutant) + UMP – ''Escherichia coli''<br />
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 **[[2j4l]] - SsUK + UTP<br />
 **[[2jjx]] - UK + ATP + Mg – ''Bacillus anthracis''<br />
-**[[1uky]] – yUK + ADP – yeast<br />
 **[[3ek5]] - XcUK + GTP <br />
-**[[3nwy]] – UK + UTP + GTP – ''Mycobacterium tuberculosis''<br />
+**[[3nwy]] – MtUK + UTP + GTP – ''Mycobacterium tuberculosis''<br />
+**[[7bex]], [[7bix]], [[7bl7]] - MtUK + UTP + UDP<br />
 **[[4a7x]], [[4a7w]] - UK + UDP – ''Helicobacter pylori''<br />
 }}