3rce: Difference between revisions

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-[[Image:3rce.jpg|left|200px]]
-<!--
+==Bacterial oligosaccharyltransferase PglB==
-The line below this paragraph, containing "STRUCTURE_3rce", creates the "Structure Box" on the page.
+<StructureSection load='3rce' size='340' side='right'caption='[[3rce]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3rce]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35221 Atcc 35221]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RCE FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
--->
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPN:PARA-NITROPHENYLALANINE'>PPN</scene></td></tr>
-{{STRUCTURE_3rce|  PDB=3rce  |  SCENE=  }}
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pglB, Cla_1253 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=201 ATCC 35221])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rce OCA], [https://pdbe.org/3rce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rce RCSB], [https://www.ebi.ac.uk/pdbsum/3rce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rce ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[https://www.uniprot.org/uniprot/PGLB_CAMLR PGLB_CAMLR]] Oligosaccharyltransferase that catalyzes the transfer of a preassembled heptasaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains, affording a beta-linked glycan to the asparagine side chain of target proteins.<ref>PMID:21677752</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Asparagine-linked glycosylation is a post-translational modification of proteins containing the conserved sequence motif Asn-X-Ser/Thr. The attachment of oligosaccharides is implicated in diverse processes such as protein folding and quality control, organism development or host-pathogen interactions. The reaction is catalysed by oligosaccharyltransferase (OST), a membrane protein complex located in the endoplasmic reticulum. The central, catalytic enzyme of OST is the STT3 subunit, which has homologues in bacteria and archaea. Here we report the X-ray structure of a bacterial OST, the PglB protein of Campylobacter lari, in complex with an acceptor peptide. The structure defines the fold of STT3 proteins and provides insight into glycosylation sequon recognition and amide nitrogen activation, both of which are prerequisites for the formation of the N-glycosidic linkage. We also identified and validated catalytically important, acidic amino acid residues. Our results provide the molecular basis for understanding the mechanism of N-linked glycosylation.
-===Bacterial oligosaccharyltransferase PglB===
+X-ray structure of a bacterial oligosaccharyltransferase.,Lizak C, Gerber S, Numao S, Aebi M, Locher KP Nature. 2011 Jun 15;474(7351):350-5. doi: 10.1038/nature10151. PMID:21677752<ref>PMID:21677752</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-[[3rce]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_lari Campylobacter lari]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCE OCA].
+<div class="pdbe-citations 3rce" style="background-color:#fffaf0;"></div>
-[[Category: Campylobacter lari]]
+== References ==
-[[Category: Aebi, M.]]
+<references/>
-[[Category: Gerber, S.]]
+__TOC__
-[[Category: Lizak, C.]]
+</StructureSection>
-[[Category: Locher, K P.]]
+[[Category: Atcc 35221]]
-[[Category: Numao, S.]]
+[[Category: Large Structures]]
+[[Category: Aebi, M]]
+[[Category: Gerber, S]]
+[[Category: Lizak, C]]
+[[Category: Locher, K P]]
+[[Category: Numao, S]]
 [[Category: Acceptor peptide]]
 [[Category: Glycosylation]]