Sandbox 5: Difference between revisions

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[[Image:1ema BIOmics 5.gif|100px| add title as well]]
==Your Heading Here (maybe something like 'Structure')==
<StructureSection load='5eqh' size='350' side='right' caption='GLUT1 transporter in complex with inhibitor (PDB entry [[5eqh]])' scene=''>


Anything in this section will appear adjacent to the 3D structure and will be scrollable.


Green fluorescent protein ('''GFP'''), originally isolated from the jellyfish Aequorea victoria (PDB entry [[1ema]]), fluorsceses green (509nm) when exposed to blue light (395nm and 475nm). It is one of the most important proteins used in biological research because it can be used to tag otherwise invisible gene products of interest and thus observe their existence, location and movement.
<scene name='10/101618/Glut1_n-glycosylation/1'>N-linked glycosylation</scene> site at Asn 45


 
</StructureSection>
== Exploring the Structure ==
<applet load='1ema' size='300' frame='true' align='right' caption='Insert caption here' />
 
GFP is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The <scene name='Sandbox_5/Green_fluorescent_protein/2'>chromophore</scene>, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The chromophore forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues.<ref>PMID:8703075</ref>
 
== References ==
 
<references/>

Latest revision as of 21:44, 25 April 2022

Your Heading Here (maybe something like 'Structure')Your Heading Here (maybe something like 'Structure')


Anything in this section will appear adjacent to the 3D structure and will be scrollable.

site at Asn 45


GLUT1 transporter in complex with inhibitor (PDB entry 5eqh)

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Student, Alexander Berchansky, Eran Hodis, Michael Skovbo Windahl, Mathilde Thomsen, Sara Toftegaard Petersen, Mette Trauelsen, Adam Kingsley
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