|
|
| (124 intermediate revisions by 7 users not shown) |
| Line 1: |
Line 1: |
| == Exploring the Structure == | | ==Your Heading Here (maybe something like 'Structure')== |
| | <StructureSection load='5eqh' size='350' side='right' caption='GLUT1 transporter in complex with inhibitor (PDB entry [[5eqh]])' scene=''> |
|
| |
|
| You can take a close look at the <font color ="green">chromophore</font> of GFP in the PDB entry [[1ema]]. The backbone of the entire protein is shown here on the left. The protein chain forms a cylindrical can (shown in blue), with one portion of the strand threading straight through the middle (shown in green). The <scene name='Sandbox_5/Gfp_erice/1'>chromophore</scene> is found right in the middle of the can, totally shielded from the surrounding environment. This shielding is essential for the fluorescence. The jostling water molecules would normally rob the chromophore of its energy once it absorbs a photon. But inside the protein, it is protected, releasing the energy instead as a slightly less energetic photon of light. The chromophore (shown in the close-up on the right) forms spontaneously from three amino acids in the protein chain: a glycine, a tyrosine and a threonine (or serine). Notice how the glycine and the threonine have formed a new bond, creating an unusual five-membered ring.
| | Anything in this section will appear adjacent to the 3D structure and will be scrollable. |
| <applet load='1ema' size='300' frame='true' align='right' caption='Insert caption here' />
| |
|
| |
|
| This picture was created with RasMol. You can create similar pictures by clicking on the accession code above and then picking one of the options under View Structure. The chromophore is called "CRO" in this file, and it is residue number 66 in the protein chain.
| | <scene name='10/101618/Glut1_n-glycosylation/1'>N-linked glycosylation</scene> site at Asn 45 |
|
| |
|
| [[Image:Erice_5.gif]]
| | </StructureSection> |